大孔吸附树脂法去除车前草粗多糖中的蛋白质  

Study on the Removal of Protein from Plantago asiatica L.Polysaccharide by Macroporous Adsorption Resin

作　　者：廖春燕[1] 杨远萍[1] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006

出　　处：《食品与发酵工业》2011年第6期193-195,200,共4页Food and Fermentation Industries

摘　　要：研究AB-8大孔树脂法去除车前草粗多糖中蛋白质的适宜条件。采用动态吸附和解析实验对树脂纯化工艺进行优化。结果表明适宜工艺条件为:上样液浓度40mg/mL,上样流速0.5 mL/min,上样液pH值7.0;以蒸馏水为洗脱剂,洗脱速度2 mL/min,洗脱体积2.5BV(1BV=20 mL)。纯化后AB-8大孔吸附树脂对车前草粗多糖中的蛋白具有较高的去除效果,蛋白去除率为84.83%,多糖保留率为88.32%。The article studied the optimal conditions of removing protein from polysaccharide of Plantago asiatica L.with AB-8 macroporous adsorption resin.The purification conditions were optimized by dynamic adsorption and desorption.The results showed that the optimum conditions were as follows：the concentration of solution was 40 mg/mL with pH of 7.0,the sample loading flow rate was 0.5 mL/min.The elution solvent was 2.5BV distilled water at flow rate of 2mL/min.AB-8 macroporous adsorption resin had the best effect,the protein removing rate was 84.83% and polysaccharide retained rate was 88.32%.

关 键 词：车前草 多糖 大孔吸附树脂 除蛋白 

分 类 号：TQ461[化学工程—制药化工]