一株高效稻草纤维素降解真菌的筛选与鉴定  

Screening and Identification of One Rice Straw-Cellulose Degrading Fungus

作　　者：熊格生[1] 刘志[2] 吴莎莎[2] 卢向阳[2] 刘如石[3] 

机构地区：[1]湖南农业大学科学技术师范学院,中国长沙410128 [2]湖南农业大学生物科学技术学院,中国长沙410128 [3]湖南师范大学生命科学学院,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2011年第4期64-68,共5页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金资助项目(31072141);长沙市科技计划资助项目(K0802157-21);湖南省高等学校科学研究资助项目(10B060);湖南省科技人才计划项目-省科技计划博士后专项资助项目(2010-2011)

摘　　要：采用刚果红羧甲基纤维素钠培养基法、滤纸条降解法和胞外酶活性测定法从长白山原始森林的腐烂枯木和落叶腐质土中筛选一株高产纤维素酶的真菌,结合菌落形态学和分子生物学方法鉴定该菌株为产黄青霉,命名为CBS-1.滤纸崩解实验说明在24 h该菌对滤纸就具有明显的崩解能力,6 d后滤纸的失重率达到24.1%;以稻草粉为底物发酵120 h后,其胞外纤维素酶活性达到33.6 mkat/L,其活性高于已经报道的菌株,是一株具有研究开发潜力的纤维素酶生产菌株.One fungi with high efficient cellulose degrading ability was isolated from the decomposed dead wood and defoliation soil of Changbai mountain primary forest, using hydrolysis spot diameter measurement method of Congo red and CMC-Na, degradation analysis method of filter paper sheet and measurement method of enzymatic activity of cellulase. The strain was identified as PeniciUium Chrysogenum with conventional morphology method as well as modem molecular biology method, named CBS-1. Filter paper sheet degradation analysis showed that strain CBS-1 had high level capacity of decomposition in 24 hours and the loss ratio of the ilter paper sheet reached 24. 1% in 6 days, and the extracellular cellulase activity of strain CBS-1 was 33.6 mkat/L when the powers of rice straw was used as the sustrates of the enzymes, which is higher than that reported in the previous reference. Therefore, strain CBS-1 was a cellulase-producing strain with broad development potential.

关 键 词：水稻秸秆 筛选 纤维素酶 降解微生物 

分 类 号：Q939.9[生物学—微生物学]