桃RAPD扩增产物的不同电泳检测及其序列特征  

Analysis of Different Electrophoresis and Sequencing of RAPD Amplification Products in Peach

作　　者：王文艳[1] 王力荣[2] 初建青[1] 杨光[1] 谭洪花[1] 宋长年[1] 房经贵[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]中国农业科学院郑州果树研究所,郑州450009

出　　处：《西北农业学报》2011年第6期141-146,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：2008年江苏省"青蓝工程"人才培育计划

摘　　要：以16个桃品种为试验材料,选用11个碱基引物以及严格筛选PCR退火温度的RAPD技术,对琼脂糖凝胶及聚丙烯酰胺凝胶电泳检测的RAPD、PCR扩增产物进行比较。结果表明,聚丙烯酰胺凝胶电泳检测出的总谱带及多态性谱带数均高于琼脂糖凝胶,前者不存在或少存在共迁移性的现象。随机选取19个片段,发现其中有6条是编码蛋白的基因片段,说明RAPD不仅扩增基因组上的非编码蛋白序列,同时可扩增编码蛋白的基因片段,能够较全面客观地反映不同桃品种遗传上的差异。In order to utilize RAPD technology better in identification of peach cultivars and genetic analysis,the RAPD fingerprints of 16 peach cultivars from agarose and polyacrylamide gel electrophoreses were compared,which was carried out with the newest optimization of RAPD technique in choosing 11 nt primers and strict screening PCR annealing temperature.The results showed that the polyacrylamide gel electrophoresis（PAGE） could detect PCR products better by presenting much more bands and more polymorphic bands could be uncovered.The random sequencing of 19 fragments showed that among which six sequences were regions of encoding protein genes,suggesting RAPD can amplify both protein-encoding gene and non-protein encoding sequences,reflecting the ability of RAPD in uncovering the genetic difference of different peach cultivars with un-bias DNA information.

关 键 词：桃 RAPD 琼脂糖凝胶电泳 PAGE 测序分析 

分 类 号：S662.1[农业科学—果树学]