低温对香樟膜脂过氧化和保护酶活性的影响  

Effect of low temperature stress on membrane lipid peroxidation and protective enzyme activity of Cinnamomum camphora

作　　者：孙存华[1,2] 孙存玉[1,3] 张亚红[1] 施剑杰[1] 孙东[1] 李百伟[1] 王丹[1] 蒋继宏[2] 

机构地区：[1]徐州师范大学生命科学学院,江苏徐州221116 [2]江苏省药用植物生物技术重点实验室,江苏徐州221116 [3]睢宁县林业指导站,江苏睢宁221200

出　　处：《广东农业科学》2011年第4期58-60,65,共4页Guangdong Agricultural Sciences

基　　金：徐州师范大学研究生科研创新项目(09YLB033);国家林业科技支撑计划项目子专题(2006BAD03A00-6)

摘　　要：以徐州引种驯化的成年香樟树叶片为材料,采用模拟低温胁迫法(温度处理分别为0℃、-5℃、-10℃、-15℃、-20℃、-25℃),研究了低温对成年香樟膜脂过氧化反应和保护酶活性的影响。结果显示,随着处理温度的降低,其叶片细胞质膜相对透性呈"S"型曲线增大,用Logistic方程计算其半致死温度为-13.40℃。叶片中MDA不断增加,SOD活性、POD活性、游离脯氨酸含量及可溶性糖含量随着处理温度的降低,呈先升后降的变化趋势。The effect of low temperature stress on membrane lipid peroxidation and protective enzyme activity in leaves of Cinnamomum camphora were studied with simulation low-temperature stress method（testing temperature was dropped from 0℃ to-25℃ gradually）in this paper.The results showed that the plasma membrane relative permeability in leaves of Cinnamomum camphora increased like a S-curve as the decreasing of temperature.Logistic equation showed that the semilethal temperature was-13.40℃.The content of MDA in the leaves increased,whereas proline,soluble sugar,the activity of SOD and POD in the leaves increased at first and then decreased as the temperature decreased.

关 键 词：低温胁迫 香樟 生理生化反应 

分 类 号：S792.23[农业科学—林木遗传育种]