微小隐孢子虫Cp735基因的克隆及原核表达  

Cloning and Prokaryotic Expression of Cp735 Gene of Cryptosporidium parvum

作　　者：范大为[1] 肖丹[1] 张西臣[1] 宫鹏涛[1] 杨举[1] 李建华[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062

出　　处：《吉林农业大学学报》2011年第5期567-570,共4页Journal of Jilin Agricultural University

基　　金：国家科技支撑计划项目(2007BAD40B05);国家传染病防治重大专项(2008ZX10004-001-B)

摘　　要：采用PCR方法从微小隐孢子虫基因组中扩增Cp735基因。与pMD-18T载体连接后,挑取阳性重组子测序分析。用基因重组技术将Cp735基因克隆入原核表达载体pET-28a中,构建Cp735基因的重组原核表达载体pET28a-Cp735。将pET28a-Cp735在大肠杆菌BL21(DE3)中诱导表达,并用SDS-PAGE和Western blot进行鉴定。结果表明:得到了在大肠杆菌中高效表达的重组蛋白,分子质量大小约为26 kD,并且具有反应原性。The Cp735 gene was amplified by PCR.Coding sequence identified by DNA sequencing was cloned into the expression vector pET-28a to construct prokaryotic expression vector.The recombinant protein was expressed in BL21（DE3）.The result of SDS-PAGE showed that the molecular weight of the expressed product was identical to that of prediction.The result of Western blot demonstrated that the expressed protein had reactogenicity.

关 键 词：微小隐孢子虫 Cp735基因 克隆 原核表达 

分 类 号：S852.7[农业科学—基础兽医学] R38[农业科学—兽医学]