Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3  

作　　者：任世英 李相前 贾建波 刘飞 肖天 

机构地区：[1]School of Life Science and Chemical Engineering, Huaiyin Institute of Technology [2]Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2011年第5期917-921,共5页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Basic Research Program of China (973 Program) (No. 2006AA10A401);the National Natural Science Foundation of China (No. 40376048)

摘　　要：Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entornophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%, 73%, and 70% similar to those from Pseudomonasfluorescens Pf-5, Marinornonas sp. MED121, and Burkholderia vietnamiensis G4, respectively. The difference of the D-hydantoinase gene between marine Halornonas sp. YSR-3 and other terrestrial organisms is distinct.Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entomophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%, 73%, and 70% similar to those from Pseudomonas fluorescens Pf-5, Marinomonas sp. MED121, and Burkholderia vietnamiensis G4, respectively. The difference of the D-hydantoinase gene between marine Halomonas sp. YSR-3 and other terrestrial organisms is distinct.

关 键 词：Halomonas sp. YSR-3 D-HYDANTOINASE polyphosphate-accumulating bacterium 

分 类 号：Q93[生物学—微生物学] Q78