D-HYDANTOINASE

作品数:4被引量:3H指数:1
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相关领域:生物学化学工程更多>>
相关机构:科尔盖特大学更多>>
相关期刊:《中国生物学文摘》《Chemical Research in Chinese Universities》更多>>
相关基金:山西省自然科学基金国家自然科学基金国家重点基础研究发展计划更多>>
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Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp. YSR-3
《Chinese Journal of Oceanology and Limnology》2011年第5期917-921,共5页任世英 李相前 贾建波 刘飞 肖天 
Supported by the National Basic Research Program of China (973 Program) (No. 2006AA10A401);the National Natural Science Foundation of China (No. 40376048)
Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoina...
关键词:Halomonas sp. YSR-3 D-HYDANTOINASE polyphosphate-accumulating bacterium 
利用重组大肠杆菌直接生产胞外酵素D-Hydantoinase
《興大工程學刊》劉姿岑(Tzu-Tsen Liu) 林家騏(Chia-Chi Lin) 吳俊彥(Jiun-Yan Wu) 劉永銓(Yung-Chuan Liu) 
生产重组蛋白以往皆利用大肠杆菌可以短时间内快速生长的特性生产目标蛋白,但是纯化蛋白质过程琐碎耗时,并且蛋白质经大量表现後也有可能会产生没有蛋白质活性(包涵体,inclusion body)的问题,增加生产的困扰。本研究运用细胞表面...
关键词:冰核蛋白 內涵肽 乙內醯尿酶 表面表現 
The immobilization of D-hydantoinase and characterization under classic condition and microwave irradiation
《中国生物学文摘》2007年第3期11-12,共2页Jia, H. H. Ni, F. Chen, M. J. Zhou, H. Wei, P. Ouyang, P. K. 
关键词:微波照射 D-乙内酰脲酶 固定术 聚苯乙烯阴离子交换树脂 D-氨基酸 
Gene Sequence, Soluble Expression and Homologous Comparison of a D-Hydantoinase fromPseudomonas putida YZ-26被引量:3
《Chemical Research in Chinese Universities》2005年第5期552-557,共6页SHI Ya-wei ZHAO Li-xia NIU Li-xi FENG Xia YUAN Jing-ming 
Supported by the Natural Science Foundation of Shanxi Province(No.20031042).
A 1440bp open-reading frame encoding D-hydantoinase from Pseudomonas putida YZ-26 was cloned and sequenced( GenBank AY387829). The DNA fragment was inserted into Nde and BamHI sites of vector pET-3a, yielding a reco...
关键词:D-HYDANTOINASE Gene sequence Soluble expression Homologous comparison PURIFICATION Mass spectrum 
Purification and Biochemical Characterization of D-hydantoinase and D-N-carbamoylase from Burkholderia cepecia.njut01
《Journal of Shanghai University(English Edition)》2005年第2期176-183,共8页李家璜 李苏平 严明 姚忠 欧阳平凯 
Hydantoinase and N-carbamoylase play important rol es in the production of optically pure amino acids from racemic 5-monosubstitut ed hydantoins. In this report, hydantoinase and the N-carbamoylase from Burkh olderia ...
关键词:D-HYDANTOINASE D-N-carbamoylase enzyme purification enzyme characterizatio n. 
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