mGM-CSF转导的小鼠黑色素瘤细胞融合巨噬细胞的抗肿瘤作用研究  

Effect of mGM-CSF modified melanoma fusion with Macrophage on tumorigencity in mice

作　　者：许进力[1,2] 王新娟[1] 

机构地区：[1]北京大学医学部,北京100083 [2]中国协和医科大学出版社,北京100730

出　　处：《癌症进展》2011年第4期448-450,403,共4页Oncology Progress

摘　　要：目的比较小鼠黑色素瘤细胞B16致瘤性与转导mGM-CSF基因的小鼠黑色素瘤细胞B16致瘤性的差别,同时研究转导该基因阳性的B16肿瘤细胞与同源小鼠巨噬细胞融合后其致瘤性变化,初步探讨巨噬细胞在肿瘤生成中的作用。方法本实验以FuGENE6介导,将融合基因Lxpsp-mGM-CSF转导逆转录病毒包装细胞PA317,以含适量感染能力的重组逆转录病毒上清液转导B16,潮霉素筛选获得阳性细胞(B16+GM-CSF),再将阳性细胞在PEG介导下与同源鼠巨噬细胞(MΦ)融合(B16+GM-CSF+MΦ)。B16、B16+GM-CSF、B16+GM-CSF+MΦ三种细胞分组分别接种于C57BL/6小鼠皮下观察成瘤能力。结果 B16组平均21天长出肿瘤;B16-GM-CSF长成实体瘤延迟7天,B16-GM-CSF-MΦ在第40天仍未长出实体瘤。结论巨噬细胞在小鼠黑色素瘤生长、转移中有重要作用。Objective To compare the tumorigencity between melanoma cell B16 and granuloeyte macrophage colony stimulating factor modified mouse melanoma cell B16, and to explore the role of macrophage in tumor growth. Methods A retrovirus mediated fusion gene of murine granuloeyte macrophage colony stimulating factor （mGM-CSF） was transferred into a package cell PA317 by FuGENE6. The virus infected the mouse melanoma B16, and the positive cells were named BI6 ＋ GM-CSF, Then the macrophage were fused with B16 ＋ GM-CSF and B16 ＋ GM-CSF ＋ M were acquired. Finally, the 3 kinds of ceils were injected into the mice. Results The tumor growed up in the mice injected with the mouse melanoma cell B16 after 21 days in average. The form of tumors in the mice with granulocyte macrophage colony stimulating factor modified mouse melanoma cell delayed for7 days. However. no tumors formed in mice injected with the fusion cells after 40 days. Conclusion Macrophages may play an important role in tumor growth and metastasis.

关 键 词：MGM-CSF 黑色素瘤B16 巨噬细胞 融合 肿瘤转移 

分 类 号：R739.5[医药卫生—肿瘤]