镉对长江华溪蟹金属硫蛋白mRNA表达的诱导及低分子量壳聚糖的联合作用  

Effects of cadmium and the combination of cadmium with low molecular weight chitosan on the mRNA expression of metallothionein in the freshwater crab Sinopotamon yangtsekiense

作　　者：周妍英[1] 李瑞金[1,2] 任国锐[1] 王兰[1] 

机构地区：[1]山西大学生命科学学院,太原030006 [2]山西大学环境科学与工程研究中心,太原030006

出　　处：《环境科学学报》2011年第9期2028-2032,共5页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金资助项目(No.30870267;30970361);山西省自然科学基金资助项目(No.2008011069);中国博士后基金资助项目(No.20100471580)~~

摘　　要：采用实时荧光定量PCR法,研究了不同浓度镉(Cd2+)对长江华溪蟹(Sinopotamon yangtsekiense)金属硫蛋白(metallothionein,简称MT)在肝胰腺、鳃、肌肉和心脏中mRNA表达的诱导作用及低分子量壳聚糖(lowmolecular weight chitosan,简称LMWC)的联合作用.镉浓度分别为29、58和87mg.L-1,镉与LMWC的联合作用组分别为58mg.L-1Cd2++20mg.L-1LMWC、58mg.L-1Cd2++40mg.L-1LMWC和58mg.L-1Cd2++80mg.L-1LMWC;处理时间均是4d.结果显示,与对照组相比,在镉胁迫下,长江华溪蟹肝胰腺、鳃和肌肉组织中MTmRNA表达显著增加,而心脏中的变化不显著.在LMWC和镉共同作用下,MTmRNA的表达在肝胰腺和鳃组织中比镉单独作用(58mg.L-1Cd2+)显著下降,而肌肉和心脏中的变化没有统计学意义.镉能诱导长江华溪蟹4种组织中MTmRNA的表达,且具有组织差异性;在LMWC与镉联合作用下,MTmRNA的表达有一定程度的下降.In order to investigate the impact of cadmium on the mRNA expressions of metallothionein（MT） in hepatopancreas,gill,heart and muscle of the freshwater crab Sinopotamon yangtsekiense and its effects in combination with low molecular weight chitosan（LMWC）,individual crabs were exposed to different concentrations of cadmium（0,29,58 and 87 mg·L-1）,or exposed to cadmium（58 mg·L-1） plus different concentrations of LMWC（20,40 and 80 mg·L-1） for 4 days.The expression levels of MT were measured by real-time quantitative PCR.The results showed that cadmium significantly induced mRNA expressions of MT in hepatopancreas,gill and muscle,while the alteration of mRNA was not significant in the heart of S.yangtsekiense compared with the control.Moreover,the mRNA expressions in hepatopancreas and gill were markedly decreased in the groups of cadmium plus LMWC compared with the cadmium group.It is concluded that cadmium can induce MT mRNA expressions in four tissues of S.yangtsekiense.However the effects are different in various tissues.The combination treatment of LMWC and cadmium can decrease the MT in S.yangtsekiense in a certain range.

关 键 词：长江华溪蟹 金属硫蛋白mRNA表达 镉 低分子量壳聚糖 

分 类 号：X171.5[环境科学与工程—环境科学]