老年根面龋牙菌斑群落组成的多样性观察  

Observation of genetic diversity in dental plaque of elder people with root caries

作　　者：马善奋[1] 梁景平[1] 姜云涛[1] 朱彩莲[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院牙体牙髓科,上海市口腔医学重点实验室,上海200011 [2]上海交通大学医学院附属第九人民医院·口腔医学院口腔微生物实验室,上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2011年第4期394-397,共4页Shanghai Journal of Stomatology

基　　金：“十一五”国家科技支撑计划(2007BAI18B01);上海市科学技术委员会生物医药项目(074119513)

摘　　要：目的:对老年人牙菌斑进行群落分析,以了解根面龋微生境构成的多样性。方法:提取25例不同龋敏感性老年人牙菌斑细菌DNA,使用PCR-DGGE技术分析细菌遗传多样性。采用SPSS12.0软件包对不同组间细菌基因型数量做方差分析。结果:分布于根龋组牙菌斑内的细菌基因型比另外2组显著增多。冠龋组与无龋组间无显著性差异。结论:老年人根龋患者牙菌斑细菌群落多样性明显高于冠龋患者及无龋者。PURPOSE： Bacterial community in dental plaque of elder people was analyzed to learn about the microhabitat composition and diversity.METHODS： Dental plaque samples were collected from 25 elders.PCR-based denaturing gradient gel electrophoresis（PCR-DGGE） was used to evaluate the microbial diversity by displaying PCR-generated 16SrDNA fragments that migrate at different distances,reflecting the different sequence of fragment.SPSS12.0 software was used to analyze the variance of genotypes between different groups of bacteria.RESULTS： Genotypes of bacteria in dental plaques in the root caries group was significantly more than the other two groups.Crown caries group and caries-free group had no significant difference.CONCLUSIONS：The genetic diversity of the dental plaque microflora in the root caries group is significantly higher than coronal caries group and caries-free group.Supported by Programme of ＂the Eleventh-Five Year＂ Plan,Ministry of Science and Technology of China（2007BAI18B01） and Bio-medical Project of Science and Technology Commission of Shanghai Municipality（074119513）.

关 键 词：根面龋 牙菌斑 细菌群落 变性梯度凝胶电泳 

分 类 号：R781.1[医药卫生—口腔医学]