cpDNA标记在马铃薯杂交亲本选配中的应用  

Application of cpDNA Marker Technique in Parents Selection and Combination of Potato Breeding

作　　者：胡祚[1] 郝大海[1] 段晓艳[1] 王兆富[1] 李周[1] 苏跃[2] 冯泽蔚[2] 李灿辉[1] 

机构地区：[1]云南师范大学薯类作物研究所,昆明650092 [2]贵州省农业厅农业技术推广总站,贵阳550001

出　　处：《种子》2011年第8期29-33,共5页Seed

基　　金：基金项目：农业部公益性（农业）科研专项（3-20）;云南省科技厅重点新产品开发计划（2009BB01）;科技创新强省计划（2008CA027）;人才培养计划（2008PY053）;云南省现代农业马铃薯产业技术体系岗位专家;贵州省省长专项资金项目[（2009）63号];贵州省高层次人才特助项目（TLIF-2007-10）经费资助.

摘　　要：亲本选配是马铃薯杂交育种工作的重点和难点。利用叶绿体DNA（cpDNA）标记方法，对两个马铃薯杂交组合（Shepody×CIP 004和PB06×CIP 004）的亲本、及其杂交实生种子后代群体的细胞质遗传背景进行了分析。结果显示：Shepody拥有T-型cpDNA标记，属于普通栽培亚种（Solanum tuberosum ssp．tuberosum）；CIP004和PB06拥有A-型cpDNA标记，均属于安第斯栽培亚种（Solanum tuberosum ssp．andigena）；在cpDNA两个标记位点（H1和H2）上，2个杂交组合实生种子后代群体的细胞质遗传背景分别与其母本完全一致，平均生育期分别与其母本接近。上述结果表明，cpDNA标记技术在马铃薯杂交亲本选配和早世代选育中具有重要的应用价值。Parents selection and combination is the initiate and important step to potato breeding program. Using chloroplast DNA （cpDNA） marker technique, the author detected cytoplast genetic background of the parents and 380 clones derived from hybrid true potato seeds of two cross combinations （ Shepody × CIP 004 n = 187 and PB 06 ×CIP 004 n = 193 ）. Results showed that Shepody harbored T-type markers of cpDNA which was subjected to the Tuberosum subspecies of Solanum tuberosum L. , but both PB 06 and CIP 004 had A-type markers of cpDNA which belonged to the Andigena subspecies of Solanum tuberosum L.. All clones tested could also be divided into two groups which separately shared the same cpDNA markers with female parents, and the averaged growth period of each group tended to female parents, respectively. The aboved results demonstrated that there was a great potential for cpDNA marker technique in parents selection and combination of potato breeding program.

关 键 词：马铃薯 亲本选配 叶绿体DNA标记 

分 类 号：S532[农业科学—作物学]