基于高密度培养的反复分批发酵法生产丁二酸  

Succinic acid production by repeated batch fermentation based on high density culture

作　　者：徐冰[1] 马江锋[1] 梁丽亚[1] 刘嵘明[1] 王光明[1] 张敏[1] 姜岷[1] 

机构地区：[1]南京工业大学生物与制药工程学院,材料化学工程国家重点实验室,江苏南京210009

出　　处：《化工学报》2011年第9期2595-2599,共5页CIESC Journal

基　　金：国家重点基础研究发展计划项目(2009CB724701);国家自然科学基金项目(20606017);江苏省“青蓝工程”~~

摘　　要：引言 丁二酸俗称琥珀酸,是三羧酸循环的中间代谢产物和厌氧代谢的终端还原产物,广泛存在于动物、植物及微生物体内。作为重要的C4平台化合物,丁二酸可用于多种大宗化学品以及生物可降解材料的制备。利用微生物发酵生产丁二酸,由于具有原料来源广泛且价格低廉、污染小、环境友好、在发酵过程中可固定CO2、能有效降低温室效应等特征，成为近年来研究的热点。Succinic acid production by repeated batch fermentation based on high density culture was studied.The result showed that E.coli could convert glucose to succinic acid in aqueous solution of glucose and a neutralizer.When cell density increased approximately to 28 g·L-1（DCW）,the aerobically grown cells were directly brought into anaerobic conditions.Glucose concentration was maintained at about 10 g·L-1 with constant flow feeding.Cells were recycled from final fermentation broth for the next batch of repeated fermentation when the last fermentation was stopped at 30 h.After two-stage fermentation and two cycles of cell reincubation,the concentration of succinic acid was 95.1 g·L^-1,79.7 g·L^-1 and 64.4 g·L^-1 respectively.The average succinic acid yield and productivity were 72.5% and 1.93 g·L^-1·h^-1 respectively,with an increase of 29.5% and 25.9%,compared with those in the two-stage fermentation.

关 键 词：丁二酸 反复分批发酵 细胞回用 

分 类 号：TQ921[轻工技术与工程—发酵工程]