反硝化聚磷污泥的基质利用及代谢途径  

Substrate utilization and metabolic mechanism of denitrifying phosphorus accumulating sludge

作　　者：刘晖[1,2] 孙彦富[1] 周康群[1] 李军[2] 陈捷美[1] 麦志宏[1] 李研[2] 

机构地区：[1]仲恺农业工程学院环境科学与工程学院,广东广州5102252 [2]北京工业大学城建学院,北京100124

出　　处：《化工学报》2011年第9期2623-2628,共6页CIESC Journal

基　　金：国家自然科学基金项目(51078008);广东自然科学基金项目(06022869;07003251);广东省教育厅项目(LYM08067)~~

摘　　要：利用A/ASBR富集反硝化聚磷菌,并利用乙酸和葡萄糖两种基质进行反硝化聚磷实验,跟踪实验过程水中的磷酸盐、硝酸盐、COD与泥中PHA和糖原的变化,研究代谢途径。结果表明:(1)厌氧时磷酸盐的释放将决定PHA的合成,缺氧时PHA的分解一方面作为碳源将NO3--N还原成了N2,同时产生的ATP为聚磷过程提供能量,因此在研究反硝化聚磷效果时,应重点跟踪ΔPHA,而不是ΔCOD。(2)以葡萄糖为基质时只有先通过发酵型的菌将葡萄糖分解为小分子的挥发性有机酸,才能充分发挥非发酵菌的反硝化聚磷功能。(3)厌氧阶段合成PHA所需要的能量ATP约85%来自磷酸盐的释放,15%来自糖原的酵解。缺氧阶段,85%的PHA分解产生的ATP用于聚磷,15%转化为糖原,因此在考虑代谢途径时可将其进行简化。The A/A sequencing batch reactor（SBR）was used to enrich denitrifying and phosphorus accumulating bacteria（DPB）.Denitrifying and phosphorus accumulating tests were performed by using acetic acid and glucose as substrate.The metabolic mechanism and change of phosphorus/nitrate/COD in water and polyhydroxyalkanoates（PHA）/glycogen in sludge were studied.The release of poly-phosphate would control the combination of PHA.At the anoxic stage breakdown of PHA was used as carbon source to denitrify nitrate,and the adenosine triphosphate（ATP）thus generated provided energy for accumulating phosphorus.So it is important to track the change of PHA,not COD when the denitrifying and phosphorus accumulating process was studied.When glucose was used as substrate,fermented bacteria broke down glucose into volatile organic acid of small molecules,then non-fermented bacteria could play a role fully.At the anaerobic stage,85% energy came from the release of phosphorus,15% energy came from the fermentation of glycogen.At the anoxic stage,85% PHA breakdown was used to accumulate phosphorus,15% was converted into glycogen.The metabolic mechanism could be simplified.

关 键 词：反硝化聚磷 代谢途径 基质利用 PHA 非发酵菌 

分 类 号：X172[环境科学与工程—环境科学]