胆总管腺癌PUMA和ABCG2的表达及其临床病理意义  

Expression of PUMA and ABCG2 and their clinicopathological correlations in adenocarcinoma of common bile duct

作　　者：华颂文[1] 杨竹林[1] 苗雄鹰[1] 

机构地区：[1]中南大学湘雅二医院普通外科,湖南长沙410011

出　　处：《中国普通外科杂志》2011年第8期818-822,共5页China Journal of General Surgery

摘　　要：目的探讨胆总管腺癌和癌旁组织中PUMA和ABCG2的表达水平及其临床病理意义。方法 40例胆总管腺癌和15例癌旁组织手术切除标本常规制作石蜡包埋切片,免疫组化法检测PUMA和ABCG2的表达。结果胆总管腺癌PUMA和ABCG2表达阳性率及其评分明显高于癌旁组织(PUMA:67.5%vs.13.3%,P=0.001,2.58±1.84vs.0.53±1.13,P=0.000;ABCG2:60.0%vs.13.3%,P=0.002,2.28±1.88vs.0.53±1.13,P=0.001)。PUMA和ABCG2表达阳性的癌旁组织上皮均呈轻至中度不典型增生;腺癌I级和淋巴结未转移病例PUMA和ABCG2表达阳性率及其评分明显低于腺癌II级或III级和淋巴结转移者(P<0.05或P<0.01)。PUMA和ABCG2在胆总管腺癌中表达评分呈高度正相关(r=0.65,P=0.000)。结论 PUMA和ABCG2表达与胆总管腺癌发生、临床生物学行为及预后有密切关系;两者的高表达预示预后不良。Objective To investigate the expression of PUMA（p53 up-regulated modulator of apoptosis） and ABCG2（ATP-binding cassette subfamily G member 2） in adenocarcinoma of common bile duct（CBD） and adjacent non-cancerous tissue and their clinicopathological correlations. Methods The surgical specimens of 40 cases of CBD adenocarcinoma and paired adjacent CBD tissue in 15 of these cases were paraffin embedded and sectioned using conventional method.The expression level of PUMA and ABCG2 was detected with immunohistochemical staining. Results The positive expression rate and scores of PUMA and ABCG2 in adenocarcinoma were significantly higher than those in adjacent tissues（PUMA： 67.5% vs.13.3%,P=0.001,2.58±1.84 vs.0.53±1.13,P=0.000;ABCG2： 60.0% vs.13.3%,P=0.002,2.28±1.88 vs.0.53±1.13,P=0.001）.The adjacent CBD tissues that showed positive expression of PUMA and ABCG2 totally presented with atypical hyperplasia of slight to moderate degree.The positive expression rates of PUMA and ABCG2 in the cases of histologic grade I and lymph node without metastasis were significantly lower than those of histologic grade II or III with involved lymph node（P〈0.05 or P〈0.01）.There was a closely positive correlation between the expression scores of PUMA and ABCG2 in CBD adenocarcinoma（r=0.65,P=0.000）. Conclusions The expression of PUMA and ABCG2 is closely correlated with the carcinogenesis and clinical biological behaviors of CBD adenocarcinoma,and the high-level expression of both proteins predicts a poor prognosis.

关 键 词：胆管肿瘤/病理学 ATP结合转运蛋白G超家族成员2 p53上调凋亡调控因子 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]