华重楼内生菌Iun35的分离及其抗菌蛋白的性质  

Isolation of Endophytic Bacterium Iun35 from Paris polyphylla var. chinensis and Properties of Its Antibiotic Protein

作　　者：雍彬[1] 张超[1] 马沁沁[1] 李佳[1] 王一丁[1] 

机构地区：[1]四川师范大学生命科学学院,成都610068

出　　处：《应用与环境生物学报》2011年第4期537-540,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.30471378)资助~~

摘　　要：从华重楼(Paris polyphylla Smith var.chinensis)块茎中分离纯化得到一株具有较强抑菌活性的内生细菌Iun35,经形态特征、生理生化特性及16S rDNA鉴定为枯草芽孢杆菌(Bacillus subtilis).将分离得到的Iun35菌株发酵培养,培养液上清经硫酸铵分级沉淀、Sephadex G-75凝胶柱层析和DEAE-32纤维素柱层析分离纯化出一种抗菌蛋白UD35.用该蛋白对多种植物病原菌进行拮抗测定,结果显示,UD35对玉米纹枯病菌(Rhizoctonia solani Kuha)、小麦赤霉病菌[Gibberelle zeae(Schw.)]等多种菌有强烈抑制作用.测定其相对分子质量约为55 000,氨基酸分析结果表明其由17种氨基酸组成.Endophytic bacteria from Paris polyphylla Smith var. chinensis Hara can produce proteins with strong antibiotic activity. In this research, an active antibiotic endophytic bacterium Iun35 was isolated from the tuber of P. polyphylla var. chinensis. The strain was classified as Bacillus subtilis based on its characteristics of morphology, physiology and biochemistry as well as the 16S rDNA analysis. After （NH4）2SO4 fractional precipitation, SephadexG-75 and DEAE-32 column chromatography, an antibiotic protein UD35 was isolated and purified from the clear supernatant of fermenting liquid. The protein UD35 was tested for antagonism toward plant-pathogenic fungi and showed strong inhibiting activity against the pathogens of Rhizoctonia solani Kuha and Gibberelle zeae （Schw.）, etc. SDS-PAGE analysis determined the molecular weight of UD35 to be about 5.5×10^3. This antibiotic protein was composed of 17 kinds of amino acids by relative analysis. Fig 3, Tab 4, Ref 16

关 键 词：华重楼 内生细菌 抗菌蛋白 分离纯化 抑菌活性 

分 类 号：Q936[生物学—微生物学] S476.1[农业科学—农业昆虫与害虫防治]