职业性三氯乙烯药疹样皮炎患者血清蛋白质差异表达分析  

Quantitative proteomic analysis on the serum of patients with medicamentose-like dermatitis induced by occupational trichloroethylene exposure

作　　者：黄振烈[1] 越飞[1] 黄汉林[1] 杨杏芬[2] 夏丽华[1] 陈慈珊[1] 邱新香[1] 黄建勋[1] 李来玉[1] 

机构地区：[1]广东省职业病防治院,广东广州510300 [2]广东省疾病预防控制中心,510300

出　　处：《中国职业医学》2011年第4期271-275,共5页China Occupational Medicine

基　　金：国家自然科学基金资助项目(30500401);广东省自然科学基金资助项目(5003556);广东省科技计划项目(73076);广东省医学科学技术研究基金项目(B2008005)

摘　　要：目的比较职业性三氯乙烯药疹样皮炎(OMDTE)患者发病急性期与治愈后的血清蛋白质表达谱。方法患者血清经前处理后,双向凝胶电泳分离蛋白质,软件分析凝胶图像,基质辅助激光解吸电离飞行时间串联质谱鉴定差异表达蛋白斑点。结果与治愈后的血清蛋白质表达谱比较,在发病急性期发现41个明显差异表达的蛋白斑点,鉴定出31个蛋白。上调的蛋白有S100钙结合蛋白A8、钙网蛋白、血清淀粉样蛋白A、亮氨酸氨基肽酶、谷胱甘肽过氧化物酶等;下调的蛋白有视黄醇结合蛋白、乙酰辅酶A羧化酶、羧肽酶N等;涉及的功能通路包括炎症反应、氧化应激、视黄醇代谢、脂肪酸代谢、激肽和过敏毒素的调节等。结论 OMDTE患者在发病急性期血清蛋白质存在明显的差异表达,鉴定的差异表达蛋白质不仅为其发病机制研究提供候选靶标,并且可能作为生物标志物应用于早期诊治。Objective To compare the proteome of the serum of patients with medicamentose-like dermatitis due to occupational trichloroethylene exposure（OMDTE）in acute and recovery stages.Methods After the samples were collected and pretreated,the expression of protein in serum was analyzed by 2-dimensional electrophoresis（2-DE） and differentially expressed protein spots were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry（MALDI-TOF-TOF/MS）.Results 31 proteins with altered modifications were separated and identified by 2-DE combined with MALDI-TOF-TOF/MS.Compared with the serum proteome in the recovery stage,proteins showed up-regulated expression in acute stage included S100 cal-cium-binding protein A8,calprotectin,amyloid related serum protein SAA,leucine aminopeptidase,plasma glutathione peroxidase,etc.However,retinol binding protein,acetyl-CoA carboxylase and carboxypeptidase N were down-regulated.The function of these proteins involved in inflammatory responses,oxidative stress,retinol metabolism,fatty acid metabolism and regulation of kinins and anaphylatoxins.Conclusion The identified proteins provided target molecules for the further study on mechanisms of OMDTE and can be used as potential biomarkers for the disease.

关 键 词：三氯乙烯 药疹样皮炎 双向凝胶电泳 质谱 血清 

分 类 号：R135.1[医药卫生—劳动卫生]