源于驴血清白蛋白的肽HP-6对造血系统的体内外作用研究  

In vivo and in vitro effect of peptide HP-6 derived from donkey serum albumin on hematopoietic system

作　　者：李昊[1] 严晟旻[1] 王浙峰[1] 沈晓妙[1] 饶平凡[1] 

机构地区：[1]福州大学生物工程研究所,福建福州350002

出　　处：《中国中药杂志》2011年第16期2259-2264,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(30973974);国家自然科学基金委主任专项基金项目(8105008);福建省科技厅重点项目(2009Y0033)

摘　　要：目的:探讨通过生物信息学手段获得的源于阿胶中的重要蛋白组分驴血清白蛋白的生物活性肽HP-6对造血系统的作用效果。方法:分离培养人骨髓有核细胞(human bone marrow nucleated cells,hBMNCs)及小鼠骨髓基质细胞(murinebone marrow stromal cells,mBMSCs),通过与不同浓度肽HP-6(0.000 15,0.001 5,0.015,0.15,1.5μmol.L-1)共培养一定时间考察肽HP-6对骨髓造血相关细胞的促增殖能力,并通过电镜观察作用后的细胞形态;建立失血性贫血与环磷酰胺致贫血2种贫血动物模型,随机分组并灌胃不同剂量(1,0.1,0.01 mg.kg-1)肽HP-6,同时设立相应的对照组灌胃等体积PBS,7 d后检测贫血动物外周血象,对环磷酰胺致贫血小鼠加测骨髓细胞(bone marrow cells,BMCs)数。结果:肽HP-6可刺激人骨髓有核细胞和小鼠骨髓基质细胞增殖且具有浓度相关性,0.15μmol.L-1肽HP-6使2种细胞均达到最大增殖率,分别为152.11%,63.52%,之后呈降低趋势;电镜结果显示与肽HP-6作用后的细胞微观形态结构正常;灌胃1 mg.kg-1肽HP-6对提高外周血血小板数量及保护环磷酰胺损伤的小鼠骨髓具有一定的功效;灌胃0.1 mg.kg-1肽HP-6对提高外周血白细胞及红细胞数量有较好的效果,还可对化疗药物作用后小鼠的外周血起到一定的保护作用;灌胃0.01 mg.kg-1肽HP-6对提高外周血血小板数量具有极佳的效果,相对增殖率可达77.65%。结论:肽HP-6可刺激造血系统相关细胞增殖,对小鼠造血功能与抵抗化疗药物损伤有一定的增强作用。Objective： By bioinformatics method,the effect in hematopoietic system of bioactive peptide HP-6,which was obtained from donkey serum albumin and is one of the major protein components from donkey-hide gelatin,was investigated.Method：Human bone marrow nucleated cells（hBMNCs） and murine bone marrow stromal cells（mBMSCs） were separated and cultured with different concentration of peptide HP-6（0.000 15,0.001 5,0.015,0.15,1.5 μmol·L-1）.The effect on promoting proliferation of cells related to hematopoiesis in bone morrow was detected and the ultrastructure of cells after treated by HP-6 was observed through transmission electron microscope.Hemorrhage anemia mouse model and anemia mouse model induced by cyclophosphamide were established,and randomly divided into peptide HP-6 groups which were administered respectively with different doses（1,0.1,0.01 mg·kg-1） by gavage,and control group which was administered with PBS by gavage.Peripheral blood components of all mice and bone morrow cells（BMC） number of mice induced by cyclophosphamide were evaluated.Result： Peptide HP-6 could concentration-related promote the proliferation of hBMNCs and mBMSCs,hBMNCs got the highest reproduction rate of 152.11% and mBMSCs also got 63.52% with the concentration of 0.15 μmol·L-1,then the reproduction rate decreased while the concentration kept increasing.The transmission electron microscope showed that ultrastructure of cells was normal after treated by HP-6.1 mg·kg-1 peptide HP-6 significantly increased peripheral platelet and protected mouse morrow injured by cyclophoshamide.0.1 mg·kg-1 peptide HP-6 significantly increased peripheral platelet with relative growth rate of 77.65 %,increased peripheral white blood cells count and peripheral red blood cells count,also could protect mouse peripheral blood after treated by chemotherapeutics.Conclusion： Peptide HP-6 could promote the proliferation of cells related to hematopoietic system,enhance mouse hemopoiesis function and the resistance to chemotherapeu

关 键 词：阿胶 驴血清白蛋白 生物活性肽 贫血 动物模型 

分 类 号：R285.5[医药卫生—中药学]