大豆子叶节高频愈伤组织诱导及其植株再生  

High Frequency Induction of Callus from Soybean Cotyledonary Node Explants and Its Plant Regeneration

作　　者：孙明杰[1] 魏玄[1] 邹丹丹[1] 赵春梅[1] 蔡春梅[1] 薛仁镐[1] 

机构地区：[1]青岛农业大学生命科学学院,山东青岛266109

出　　处：《大豆科学》2011年第4期546-551,共6页Soybean Science

基　　金：国家转基因生物新品种培育科技重大专项资助项目(2008ZX08010-002;2009ZX08010-009B)

摘　　要：以萌发7 d的大豆子叶节切断及其切片为外植体,研究了萌发培养基、不同激素及其配比对大豆子叶节愈伤组织诱导与分化的影响。结果表明：当不添加BA时,在子叶节切断处几乎无愈伤组织的产生,只有添加BA时,才能产生愈伤组织。外植体切取方式、愈伤组织诱导培养基中BA和TDZ的浓度配比对愈伤组织诱导率产生很大影响。BA浓度为0.05~0.15 mg·L-1,TDZ浓度为0.04~0.12 mg·L-1时,子叶节切断外植体的愈伤组织诱导率较低,平均不到20%;BA浓度为0.15 mg·L-1和TDZ浓度为0.08 mg·L-1时,愈伤组织诱导率最高,达到50%;而以子叶节切片为外植体时,愈伤组织诱导率得到极大提高,平均达到85%以上,最高可达95%。将诱导的愈伤组织转到含有不同浓度BA的分化培养基上诱导不定芽发生,BA浓度为0.2 mg·L-1时,不定芽诱导率最高可达21.4%。Effects of germination medium and different concentration ratios of BA and TDZ on the callus induction and plant regeneration were studied using fragments and slices of 7 day-germinated soybean cotyledonary nodes as explants.The result showed that the callus formation occurred from the cotyledonary node fragments only when it was cultured on germination medium containing BA.There were significant differences in callus induction frequencies among different explants,different concentration ratios of BA and TDZ on callus induction medium.The induction frequency of callus was low for cotyledonary node fragment explants（average less than 20%）under the combination concentration of BA（0.05-0.15 mg·L-1）and TDZ（0.04-0.12 mg·L-1）,the highest induction frequency（50%） was obtained when cotyledonary node fragments were cultured on callus induction medium containing 0.15 mg·L-1 BA and 0.08 mg·L-1 TDZ.However,the induction frequency of callus was significantly enhanced by using the cotyledonary node slices as explants（average more than 85%）,the highest frequency reached 95%.The callus induced was transferred to differentiation medium containing BA for shoot induction.The highest induction frequency of shoots（21.4%）was obtained on differentiation medium containing 0.2 mg·L-1BA.

关 键 词：大豆 子叶节切断 子叶节切片 愈伤组织 植株再生 

分 类 号：S565.1[农业科学—作物学]