大豆凝集素基因Le2克隆及转化  

Cloning and Transformation of Soybean Lectin Gene Le2 into Tobacco

作　　者：方标[1] 王丕武[1] 于雷[1] 卢春蕾[1] 

机构地区：[1]吉林农业大学农学院,吉林长春130118

出　　处：《大豆科学》2011年第4期706-709,共4页Soybean Science

基　　金：国家转基因专项资助项目(2011ZX08004-004);大学生科技创新基金资助项目

摘　　要：大豆凝集素对提高植物的抗虫性有重要作用。应用基因工程手段,克隆大豆凝集素基因Le2,构建植株表达载体pSy-Le2,将大豆凝集素Le2基因通过农杆菌介导法导入烟草中,经过筛选获得转基因植株,为进一步鉴定Le2的基因功能提供了试验基础。Soybean lectin（Soybean agglutinin） is a major protein in soybean,which plays an important role in plant insects resistance as a main anti-nutritional factors.In this study,the soybean lectin gene Le2 had been cloned by genetic engineering technology,then constructed the recombinant plant expression vector pSy-Le2 and transformed it into tobacco by Agrobacterium mediated way.Transgenic plant had been selected,which supplied basic materials for further experiments.

关 键 词：大豆凝集素 基因工程 抗虫性 农杆菌 烟草 

分 类 号：S565.1[农业科学—作物学]