甘蓝型春油菜小孢子培养技术研究及其在杂交种选育中的应用  

Studies on microspore culture technology and application in hybrid breeding of spring Brassica napus L.

作　　者：星晓蓉[1] 

机构地区：[1]青海大学农林科学院青海省春油菜遗传改良重点实验室,青海西宁810016

出　　处：《青海大学学报（自然科学版）》2011年第4期5-9,共5页Journal of Qinghai University(Natural Science)

基　　金：国家科技支撑计划(2010BAD01B03)

摘　　要：以双低甘蓝型春油菜品系及其杂交F1为供体材料,对提高小孢子培养效率的因素进行了研究。结果表明:小孢子发育时期与花蕾形态具有一定的相关性,即花蕾大小在2.5～3.5mm时,80%以上小孢子处于单核晚期-双核早期,并且该时期的小孢子最易诱导成胚;供体材料播种在恒定低温条件下更利于产胚;4℃低温处理30 d能显著提高胚状体的成苗率;B5固体培养基中琼脂质量浓度为12 g/L时,诱导成苗率最高。利用小孢子培养方法选育出了青杂6号和青杂7号的恢复系。Studies on microspore culture of double-low spring lines and their F1 hybrids of Brassica napus.indicate that the certain relevance exists between the developing stage of microspore and bud size,and more than 80% of the microspore is in the single nucleus later period to binuclear early time when the bud size is 2.5~3.5mm.The embryo can be obtained easily in this stage.Donor materials are more conducive to produce embryos when planted in the constant low temperature.Plantlet rates can be enhanced through low temperature（4 ℃）and 30 d treatment.The best agar content of media is 12 g/L.The restorers of Qingza No.6 and Qingza No.7 was obtained by microspore culture.

关 键 词：甘蓝型春油菜 小孢子培养 恢复系 杂交种 

分 类 号：S33[农业科学—作物遗传育种] S565.4[农业科学—农艺学]