二化螟线粒体cox1基因的克隆、序列测定和分子系统学分析  

Cloning,Sequencing and Molecular Phylogenetic Analysis of the Mitochondrial Cytochrome Oxidase I Gene of Chilo suppressalis

作　　者：汪爱民[1] 洪桂云[1,2] 魏兆军[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]安徽建筑工业学院环境工程系,安徽合肥230601

出　　处：《安徽农业科学》2011年第21期12719-12721,共3页Journal of Anhui Agricultural Sciences

基　　金：教育部新世纪优秀人才基金项目(NCET-07-0251);安徽省优秀人才基金项目(08040106803)

摘　　要：[目的]克隆并分析二化螟线粒体细胞色素c氧化酶亚基I基因(cox1)。[方法]利用PCR方法扩增了二化螟线粒体cox1基因,并测定了其全序列。通过检索GenBank数据库获得了其他21种鳞翅目昆虫的cox1序列,并进行了同源性比较和分子系统学分析。[结果]cox1基因编码框包含1531个核苷酸,编码510个氨基酸的蛋白;起始密码子为CGA,终止密码子仅由一个T组成。利用ML方法构建了基于cox1基因编码氨基酸序列的鳞翅目昆虫的分子系统树,发现分子系统树与从形态学角度的系统分类在大方面上是基本一致的,但也略有差异。[结论]为进一步研究cox1基因的表达和应用奠定了基础。[Objective]The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene（cox1） of Chilo suppressalis.[Method]The mitochondrial cox1 gene of Chilo suppressalis was cloned with PCR method and then sequenced.Then,cox1 sequences of other 21 Lepidopteran species were obtained by blasting the GenBank with cox1 gene sequence of C.suppressalis.Finally,homology comparison and molecular phylogenitic analysis among the 22 Lepidopteran species were conducted.[Result]The opening reading frame of cox1 gene from C.suppressalis contained 1 531 nucleotides encoding a putative protein of 510 amino acids.The cox1 gene used a start codon CGA,and an incomplete termination codon composed of only T.Based on the amino acid sequences of cox1,the molecular phylogenetic tree of Lepidoptera was reconstructed using the maximum likelihood（ML） method.The molecular phylogenetic tree was similar to the morphological phylogenetic tree mainly,but also showed some differences.[Conclusion]The result will provide reference for further research on expression and application of the cox1 gene.

关 键 词：线粒体DNA 二化螟 cox1基因 系统发育分析 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]