Sry基因和Y染色体重复序列在牛早期胚胎性别鉴定中应用效果的比较  

Comparison of the Effects of Nest PCR System Established by Sry Gene and Y Chromosome Repeated Sequence for Sex Determination of Bovine Embryo

作　　者：张伟[1] 王世银[2] 张兆旺[3] 赵兴绪[4] 

机构地区：[1]新疆农业职业技术学院动物科技学院,昌吉831100 [2]新疆农业职业技术学院继续教育学院,昌吉831100 [3]甘肃农业大学动物科学技术学院,兰州730070 [4]甘肃农业大学动物医学院,兰州730070

出　　处：《中国奶牛》2011年第16期5-8,共4页China Dairy Cattle

基　　金：甘肃省科技厅资助项目<家畜胚胎规模化生产及相关新技术的产业化开发>(2GS035-A41-001-01)

摘　　要：本试验以牛Sry基因和Y染色体重复序列作为雄性特异性基因,分别设计引物,建立多重巢式PCR体系,比较二者在牛早期胚胎性别鉴定中的应用效果。试验结果表明,当扩增体系中的模板量为一个胚胎细胞的DNA量时,以Y染色体重复序列构建的扩增体系比Sry基因具有更高的灵敏度和稳定性,更适合用于牛早期胚胎性别鉴定。Sty gene and Y chromosome repeated sequence were selected as male-specific gene for sex determination of bovine embryo in this study. Eight pairs of primers were designed and a nest PCR system was established in order to compare the amplification effect. The results showed that the system established by Y chromosome repeated sequence was more sensitive and stable than the one established by Sty gene when the PCR template was the DNA from one embryonic ceil, so it was more suitable for sex determination of bovine embryo.

关 键 词：SRY基因 Y染色体重复序列 胚胎性别鉴定 牛 PCR法 

分 类 号：S823.3[农业科学—畜牧学]