螺旋藻混合营养培养中碳源分析  

Analysis on carbon source in mixotrophic culture of S.platensis

作　　者：田华[1] 远凌威[1] 张义明[2] 

机构地区：[1]信阳师范学院生命科学学院,信阳464000 [2]贵州工业职业技术学院,贵阳550008

出　　处：《中国食品添加剂》2011年第4期79-82,共4页China Food Additives

基　　金：国家自然科学基金项目(30460039);河南省科技厅科技发展计划项目(112102210309);河南省教育厅自然科学研究计划项目(2011B180051);信阳师范学院青年骨干教师资助计划

摘　　要：对螺旋藻混合营养培养过程中无机碳HCO3-和有机碳源葡萄糖进行详细的检测及分析,结果表明:在培养3d时各组所添加的葡萄糖基本用完,培养9d时,葡萄糖浓度为3.0g/L组培养液HCO3-含量最低。同时优选了螺旋藻混合营养培养的NaHCO3添加量,研究发现NaHCO3含量对螺旋藻混合营养细胞积累有很大的影响,NaHCO3的浓度在8.4g/L 16.8g/L时基本对螺旋藻生物量的积累没有太大的影响。Carbon source of inorganic carbon sodium bicarbonate and organic carbon source glucose in mixotrophic culture of S.platensis were analyzed.The results showed that glucose were almost used on the third day;on the ninth day,HCO-3concentration was at the lowest of 3.0g /L.At the same time,sodium bicarbonate concentration in mixotrophic culture of S.platensis was optimized.We found that sodium bicarbonate concentration significantly affected the biomass of S.platensis,the appropriate concentration range was 8.4g/L16.8g/L.

关 键 词：螺旋藻 混合营养培养 碳酸氢钠 葡萄糖 

分 类 号：TS202.1[轻工技术与工程—食品科学]