TGF-β_1对钛表面成骨相关蛋白基因表达的影响  

Effect of TGF-β_1 on gene expression related with osteogenesis of osteoblasts on titanium disc surfaces

作　　者：郑纪伟[1] 韩斐斐[2] 王瑾[2] 韩建国[1] 丁志江[1,3] 

机构地区：[1]徐州医学院口腔医学院,硕士生江苏221004 [2]徐州医学院临床学院,讲师江苏221004 [3]上海同济大学,在读博士研究生江苏221004

出　　处：《中华老年口腔医学杂志》2011年第2期65-69,共5页Chinese Journal of Geriatric Dentistry

基　　金：徐州医学院科研课题(编号:2010KJ09)

摘　　要：目的:通过观测特定浓度的转化生长因子β1(transforming growth factorsβ1,TGF-β1)对钛表面成骨细胞中骨钙素(osteocalcin,OC)、骨涎蛋白(bone sialoprotein,BSP)和I型胶原蛋白(collagen-I,COL-I)基因表达的影响,以探讨其对钛表面成骨细胞分化的作用。方法:体外培养成骨细胞接种于钛片表面,将其分为实验组和对照组,实验组加入0.5ng/ml的TGF-β1刺激细胞增殖和分化,收集第1d、3d、7d培养的细胞。通过RT-PCR检测不同培养时间OCmRNA、BSPmRNA和Col-ImRNA表达情况,对照组不加TGF-β1。结果:浓度为0.5ng/ml的外源性TGF-β1加入到接种于钛片表面的成骨细胞中后,均可显著增加几种细胞因子(OC、BSP和Col-I)基因的表达(P<0.05)。结论:浓度为0.5ng/ml的外源性TGF-β1对钛片表面的成骨细胞成骨相关因子表达有一定的促进作用。Objective：To investigate the consequential changes of gene expression（OC,BSP and Col-I）of osteoblasts on Titanium disc surfaces incubated with TGF-β1（0.5ng/ml）,and to make a primary understanding of functions of differentiation.Methods：Osteoblast cells were cultured in vitro,then seeded them on the surface of the Tiatanium disc.These were divided into two groups：the control group and the induced group.TGF-β1（0.5ng/ml） is administrated to induced group while control group without this treatment.The expression of mRNA were detected by reverse transcription-polymerase chain reaction（RT-PCR） after 1,3,7 days.Results：The extraneous TGF-β1（0.5ng/ml） can increase the gene expressions（OC,BSP and Col-I） of osteoblasts on Ti disc surfaces（P0.05）.Conclusions：It is concluded that extraneous TGF-β1（0.5ng/ml）can promote the differentiation of the osteoblast cells on the surface of the Tiatanium disc.

关 键 词：转化生长因子Β1 钛 成骨细胞 逆转录聚合酶链反应 

分 类 号：R783.1[医药卫生—口腔医学]