实时荧光定量检测大肠癌患者血浆miR-21的表达  

Detection of plasma circulating miR-21 of colorectal carcinoma patients by aquantitative real-time PCR

作　　者：孙建斌 夏琳[2] 

机构地区：[1]武汉市第三医院检验科,武汉430060 [2]武汉大学中南医院基因诊断中心

出　　处：《临床血液学杂志（输血与检验）》2011年第4期444-447,共4页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:研究大肠癌患者血浆miR-21表达与临床病理的关系。方法:用实时荧光定量方法(qRT-PCR)检测70例大肠癌和20例良性大肠疾病患者,以及25名健康对照血浆miR-21表达,同时检测大肠癌患者血浆癌胚抗原(CEA)含量。结果:70例大肠癌患者中,50例miR-21表达阳性,阳性率71.4%,20例良性大肠疾病阳性率为5.0%(1/20),大肠癌患者与良性大肠疾病和健康对照组血浆miR-21比较差异有统计学意义(χ2=24.42,P<0.01)。大肠癌患者血浆miR-21表达在不同性别(χ2=0.113,P=0.735)、年龄(χ2=0.113,P=0.735)、Dukes分期(χ2=1.525,P=0.679)、肿瘤部位(χ2=2.284,P>0.05)间差异无统计学意义。70例大肠癌患者血浆miR-21表达阳性率与CEA阳性率(63.4%)比较,差异无统计学意义(χ2=2.280,P>0.05)。结论:qRT-PCR是一种快速、准确、定量检测miR-21表达的方法,大肠癌患者血浆miR-21是一项有效的辅助诊断大肠癌的分子标志。Objective：To investigate the expression of plasma miR-21 of colorectal carcinoma patients and the relationship between the plasma miR-21expression and clinicopathological features.Method：Peripheral blood samples were collected from 70colorectal carcinoma patients,20benign colorectal disease patients and 25normal controls.The plasma miR-21was measured by quantitative real-time PCR（qRT-PCR）,and CEA of colorectal carcinoma patients was measured by chemiluminescence immunoassay.Result：The qRT-PCR method was highly both accurate and sensitive in measuring miR-21.Regarding plasma expression of colorectal carcinoma patients,50out of 71（71.4%）were found to be positive.The difference of miR-21expression between benign colorectal diseases and cancer patients was significant（χ2=24.42,P0.001）.When the gender,age,Dukes stage and tumor site of patients with colorectal carcinoma were evaluated,there were not significant differences of miR-21expression.The positive rate of CEA in colorectal carcinoma patients was not significantly higher than that of miR-21expression （χ2=2.284,P=0.124）.Conclusion：The qRT-PCR method would be a useful tool to rapidly and precisely quantify telomerase gene expression,and plasma miR-21of colorectal carcinoma patients might be a simple and useful molecular marker for the diagnosis of colorectal carcinoma.

关 键 词：大肠癌 MIR-21 QRT-PCR 

分 类 号：R735.3[医药卫生—肿瘤]