肺癌细胞裂解物负载对树突状细胞分泌的exosome诱导抗肿瘤作用的影响  

Anti-tumor effect induced by exosomes derived from dendritic cells loaded with lung cancer cell lysates

作　　者：张在云[1] 李希德 刘叶[1] 王志仑[1] 潘祥林[1] 

机构地区：[1]山东大学第二医院,济南250033 [2]高密县中医院

出　　处：《山东医药》2011年第31期4-6,共3页Shandong Medical Journal

基　　金：山东省自然科学基金资助项目(Y2007C111)

摘　　要：目的为制备高效的胞外体(exosome)肿瘤疫苗提供理论依据。方法用细胞因子诱导培养树突状细胞(DC),将肺癌细胞裂解物负载DC,提取exosome;用exosome活化T细胞(负载组),以未负载DC的exosome(未负载组)及肺癌细胞裂解物负载DC(DC组)活化的T细胞为对照,MTT法检测三组肺癌细胞的杀伤率。结果 ex-osome中有HSP70、HLA及CEA表达。活化T细胞/肺癌细胞为25∶1、10∶1、5∶1时负载组杀伤率均明显高于未负载组及DC组(P均<0.05)。结论肺癌细胞裂解物负载能增强DC分泌的exosome诱导的抗肿瘤作用;本研究为制备高效的exosome肿瘤疫苗提供了理论依据。Objective To obtain theoretical bases for making high efficacy exosome cancer vaccine. Methods Dendritic cells （DC） were induced with cytokines and then loaded with whole lung cancer cell lysates. Exosomes were isolated from supematant of DC, and T cells activated by the exosomes （ group loaded）, T cells activated by exosomes from non-loaded DC （group non-loaded） or activated by lysate-loaded DC（ group DC） were taken as control. The activity of T cells for killing lung cancer cells were detected by MTr method. Results HSP70, HLA and CEA protein were found in exosomes. The kill rates of activated T cells in group loaded at E： T ratio 25： 1, 10： 1, 5：1 were much higher than those in group non-loaded and group DC （ all P 〈 0.05）. Conclusions Lung cancer cell lysates loading can promote the anti-tumor activity induced by DC-defived exosomes ; this study can provide theoretical bases for making high efficacy exosome cancer vaccine.

关 键 词：肺肿瘤 树突细胞 胞外体 肿瘤疫苗 

分 类 号：R734.2[医药卫生—肿瘤]