Th1/Th2细胞模式与肿瘤相关性的探讨  

Investigation on the correlation between Th1/Th2 cell model and tumor

作　　者：王长印[1] 李萍[1] 董振芳[1] 卢志明[1] 

机构地区：[1]山东大学附属省立医院,济南250021

出　　处：《山东医药》2011年第31期25-26,共2页Shandong Medical Journal

基　　金：济南市高校自主创新计划项目(200906050)

摘　　要：目的观察Th1/Th2模式与肿瘤的相关性及细胞因子对Th1/Th2分化的影响,为肿瘤的免疫治疗提供依据。方法选择胃癌患者78例(胃癌组)和健康体检者80例(对照组),采用酶联免疫斑点法检测两组Th1、Th2、Th1/Th2水平;对照组35例行外周血单个核细胞培养并分为A、B、C组,A、B组培养过程中分别加入IL-12+抗IL-4单抗(终浓度分别为4μg/L和10 mg/L)、IL-4+抗γ-IFN单抗(终浓度分别为20μg/L和10 mg/L),C组不干预,检测各组Th1/Th2细胞的比率。结果胃癌组中Th1明显低于、Th2明显高于对照组,Th1/Th2明显低于正常组,P均<0.05;与C组比较,A组Th1细胞阳性率升高,Th2细胞阳性率降低,Th1/Th2值升高;B组Th1细胞阳性率降低,Th2细胞阳性率升高,Th1/Th2值降低(P<0.05)。结论胃癌患者血清呈现Th2优势的分化状态;IL-12+抗IL-4单抗可改变此状态;此为进一步提高肿瘤的免疫治疗效果提供了理论依据。Objective To observe the correlation between Th1/Th2 cen model and tumors, and the influence of cyto- kines on Th1/Th2 paradigm, so to provide basis for the immunotherapy of tumor. Methods Seventy-eight gastric cancer patients（ gastric cancer group） and 80 health controls （ control group） , were enrolled in , enzyme-linked immunospet assay was used to measure Thl, Th2, Thl/Th2 paradigm, the peripheral blood mononuclear cells of 35 cases in control group were cultured and divide into A, B, C group, during the course of culture IL-12 ＋ anti-IL-4 monoclanal antibody （last concentration 4 μL and 10 rag/L） and IL-4 ＋ anti-γ-IFN monoclonal antibody（last concentration of 20 μL and 10 mg/ L） were added to A and B group, the C group was not intervened, and the ratio of Thl/Th2 was detected. Results Compared with the control group,Thl and the ratio of Thl/Th2 in the gastric cancer group apparently decreased,Th2 apparently increased, all P 〈0.05. Compared with the C group, the percentage of Thl cells and Thl/Th2 mtioin in group A were higher and the percentage of Th2 cells were lower, the percentage of Thl cells and Th1/Th2 ratio in group B were lower and the percentage of Th2 cells were higher （ P 〈 0.05）. Conclusions Patients with gastric cancer are in the condition of Th2 predominance; IL-12 and anti-IL-4 monoclonal antibody may change this status.

关 键 词：胃肿瘤 胃癌 Th1/Th2细胞模式 细胞因子 

分 类 号：R735.2[医药卫生—肿瘤] R446.6[医药卫生—临床医学]