N^+注入诱变选育菜粕硫苷降解菌的研究  

A Study on the Breeding by Ion Implantation and Cultivation of the Rapeseed Meal Glucosinolates Degrading Strain

作　　者：景志远[1] 李吕木[1,2] 丁小玲[1,2] 许发芝[1,2] 刘海进[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽省畜牧生物工程技术研究中心,安徽合肥230031

出　　处：《激光生物学报》2011年第4期468-473,共6页Acta Laser Biology Sinica

基　　金：国家科技部科技人员服务企业行动项目(2009GJC30021);安徽省农业科技成果转化项目(09150306006)

摘　　要：为选育能够高效降解菜粕硫苷(Glucosinolates,Gls)的菌株,利用能降解硫苷的益生菌黑曲霉CICC-2238进行N^+注入诱变。经过两次N^+注入诱变,在诱变能量为20 keV,剂量为60×2.5×10^(13)ions/cm^2时,筛选到一株高效降解Gls的菌株H-109。与出发菌相比较,其Gls的降解率由28.95%提高到49.88%。结果表明,N^+注入诱变可以用于菜粕硫苷高效降解菌的选育。To obtain an efficient rapeseed meal glucosinolates degrading strain by N ~ ion implantation. Aspergillus ni- ger CICC-2238 was original probiotic, which was implanted by N ~ ion with dose of 60 ×2.5 × 1013 ions/cm2 under im- plantation energy for 2 times and a high-yield mutant strain H-109 was obtained. Compared with the original strain, the glucosinolate degradation rate of mutant strains was enhanced from 28.95% to 49.88%. The results showed that N ~ ion implantation mutation was a successful approach of breeding rapeseed meal glucosinolates degrading strain.

关 键 词：菜粕 硫代葡萄糖苷 黑曲霉 N+离子注入 诱变育种 

分 类 号：Q691[生物学—生物物理学]