TGF-β1基因SNPs与长沙汉族人群脑卒中的相关性研究  

A Research on the Relationship between the TGFBl SNPs and Stroke in Han People in Changsha Area

作　　者：梁辉[1] 杨期东[2] 曾凯敏[1] 

机构地区：[1]湖南省人民医院神经内科,湖南长沙410002 [2]中南大学湘雅医院神经内科,湖南长沙410008

出　　处：《激光生物学报》2011年第4期523-529,共7页Acta Laser Biology Sinica

摘　　要：目的:通过对长沙汉族人群TGFB1的多态分布规律的研究,从遗传流行病学的角度探讨TGFB1 SNPs(单核甘酸多态性)与长沙汉族人群脑卒中的关系。方法:应用PCR、RFLP及DNA直接测序等方法对研究人群进行-509C>T及+869T>C基因分型。研究对象包括:脑梗死(CI)患者186例,脑出血(CH)患者202例,正常对照人群160例。结果:脑梗死组(CI)和脑出血组(CH)分别与对照组比较,-509C>T和+869T>C基因型及等位基因频率分布无统计学差异(P>0.05),有脑梗死家族史的患者(FCI组)与对照组比较,-509 T等位基因携带者及+869C等位基因携带者频率较高(P<0.05),其中-509 T携带者脑梗死的患病风险为对照组的1.557倍,+869C携带者脑梗死的患病风险为对照组的1.45倍。结论:TGFB1-509C>T及+869T>C与有脑梗死家族史的长沙汉族人群脑梗死发病可能相关,但与有脑出血家族史的长沙汉族人群脑出血发病无关,-509T和+869C等位基因可能是有脑梗死家族史的长沙汉族人群脑梗死发病的危险因子。Abstract： OBJECTIVE：Through researching of the TGFB1 SNP of Han population in Changsha area, tile relationship between the TGFB1 SNPs and the stroke in this area has been explored. METHOD ：The genotypes of 186 patients with cerebral infarction, 202 patients with primary cerebral hemorrhage and 160 sex- and age-matched heath controls from Hart population in Changsha area were detected by PCR-RFLP and direct DNA sequencing. RESULT：The frequencies of the -509C 〉 Tand ＋ 869T 〉 C genotypes and alleles showed no difference between the CI and the control groups, and no difference was found between the CH and the control groups （ P 〉 0.05 ） , either. But the frequencies of -509T and ＋ 869C carriers in FCI were higher than control group. The stroke prevalences of the -509T carriers in FCI were 1. 557 multiples compared to the control group, while the ＋ 869C carriers in FCI were 1.45 multiples compared to the control group. CONCLUSION： TGFB1 -509C 〉 T and ＋ 869T 〉 C are associated with cerebral infarction of people with a familyhistory of cerebral infarction, but have no relationship with cerebral hemorrhage. -509 T and ＋ 869C alleles are probably the risk factors of stroke of people with history of cerebra/infarction.

关 键 词：转换生长因子一β1 单核甘酸多态性 脑梗死 脑出血 

分 类 号：R743.3[医药卫生—神经病学与精神病学]