大蒜多糖预处理对缺氧缺糖/复氧复糖大鼠心肌细胞的拮抗作用  

Inhibition of garlic polysaccharide pretreatment on cultured myocardiocytes injury induced by oxygen and glucose deprivation /reoxygenation

作　　者：余薇[1] 吴基良[1] 查文良[1] 李璐[1] 姚社[1] 

机构地区：[1]咸宁学院药学院,437100

出　　处：《实用医学杂志》2011年第18期3288-3290,共3页The Journal of Practical Medicine

摘　　要：目的:研究大蒜多糖(GP)预处理对缺氧缺糖/复氧复糖损伤大鼠心肌细胞的拮抗作用研究,并初步探讨其作用机制。方法:采用连二亚硫酸钠(Na2S2O4)建立乳鼠心肌细胞糖氧剥离的A/R损伤模型,不同剂量GP(10、30和100mg/L)预处理24h后,比色法测定细胞培养液中iNOS和NO含量,测定细胞内SOD活性及MDA含量。结果:与A/R模型组比较,GP预处理可增加胞内SOD活性和培养液中iNOS活力,降低NO释放量和胞内MDA含量。结论:GP预处理对A/R损伤心肌细胞具有明显的抗氧化作用,可能与清除自由基的产生有关。Objective To investigate the inhibition of garlic polysaccharide pretreatment on cultured myoeardiocytes injury induced by oxygen and glucose deprivation /reoxygenation （A/R） and its mechanisms. Methods The model of oxygen and glucose deprivation/reoxygenation injury was developed by Na2S2O4 in cultured neonatal rat cardiomyoeytes.The cuhured cardiocytes were pretreated with different concentrations of garlic polysaccharide （10,30 and 100mg·L-1）betbre anoxia/reoxygenation. The activities of SOD, iNOS, and the contents of MDA and NO were determined by colorimetry respectively. Results Compared with A/R model group, garlic polysaccharide could significantly increase the activity of SOD, inhibit the activities of iNOS in supernatant, decrease the contents of MDA and NO in a concentration dependent manner. Conclusion Garlic polysaccharide has a protective effect on myocardiocytes against anoxia/reoxygenation injury and the mechanism may be related with its oxygen free radical scavenging ability and regulation of the NO contents.

关 键 词：大蒜多糖 心肌细胞 糖氧剥离/复氧 NO 抗氧化 

分 类 号：R285[医药卫生—中药学]