心理应激对大鼠颞下颌关节内白介素-1白介素-6表达的影响  

The influence of emotional stress to the IL-1 and IL-6 of the temporomandibular joints in rats

作　　者：吴高义[1] 陈磊[2] 孙晶[1] 黄飞[1] 朱国雄[1] 李冬临[1] 

机构地区：[1]济南军区总医院口腔科,济南250031 [2]济南市口腔医院正畸科

出　　处：《中华行为医学与脑科学杂志》2011年第8期685-687,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：第48批中国博士后科学基金项目（20100481522）,济南军区总医院院长基金项目（2010M08）

摘　　要：目的通过对心理应激大鼠颞下颌关节（tempommandihular joint，TMJ）的形态、生理学评估及相关炎性因子的检测，探讨心理应激对TMJ的可能致病机制。方法采用交流箱心理应激动物模型，检测空白对照组、心理应激组（PS组）、心理应激加地西泮组（Ps＋DI组）大鼠血清中Cor和ACTH水平；运用RT—PCR技术检测心理应激不同时期大鼠TMJ内炎性因子IL-1和IL-6的表达变化。结果空白对照组血清中Cor和ACTH值与PS组有显著差异（P〈0．01），空白对照组血清中Cor和ACTH值与PS＋DI组无显著差异（P〉0．05）；空白对照组IL-1（分别为（0．453±0．021）mg／L，（0．439±0．028）mg／L，0．454±0．023）mg／L、IL-6[分别为（0．525±0．028）mg／L，（0．515±0．028）mg／L，（0．518±0．022）mg／L]与PS组IL-1分别为（0．981±0．024）mg／L，（0．746±0．017）mg／L，（0．510±0．016）mg／L]、IL-6[分别为（0．820±0．023）mg／L，（0．694±0．019）mg／L，（0．579±0．015）mg／L]有显著差异（P〈0．05），与PS＋DI组IL-1[分别为（0．549±0．014）mg／L，（0．498±0．014）mg／L，（0．444±0．022）mg／L]、IL-6[分别为（0．5998±0．015）mg／L，（0．541±0．015）mg／L，（0．487±0．008）mg／L]差异无显著性（P〉0．05）。结论心理应激可能在TMD形成过程中起着重要作用。Objective To investigate the possible mechanisms of the attribution of psychological stress to the temporomandibular joint disorder（TMD）, through the evaluation of the animal model and detection of the proinflammatory cytokines in the TMJ. Methods The animal models of communication box were built to mimic the psychological stress. The concentration of the serum Cot and ACTH was detected in the control group,Psychological Stress group （ PS group）, and diazepam （ anti-anxiety drug） group （ PS ＋ DI group）. The expression of the proinflammatory cytokines IL-1 and IL-6 in the rat TMJ in the different phases of psychologicalstress was detected by RT-PCR. Results The results of the serum concen- tration of Cor and ACTH showed that there was significant difference between the control group and the PS group（P 〈 0. O1 ） ,while no signifcant difference between the control group and the PS ＋ DI group （P 〉 0.05 ）. The expressions of IL-1 and IL-6 were compared in all group. The expressions of IL-1 in CON group were （0. 453 ± 0. 021 ） mg/L, （0. 439 ± 0. 028 ） mg/L and （0. 454± 0. 023 ） mg/L. These values were markedly increased compared with those of the PS group（0. 981 ± 0. 024）mg/L, （0.746 ±0.017 ） mg/L and （0.510 ±0.016 ） mg/L respectively, P 〈 0.01 ）, hut no significant differences compared with PS ＋ DI group（0. 549 ± 0. 014） mg/L, （0. 498 ± 0. 014） mg/L and （0. 444 ± 0. 022） mg/L respectively, P 〉 0.05 ）. Similar changes were observed in expressions of IL-6. The expressions of IL-6 in the CON rats were （0. 525 ± 0. 028 ） mg/L, （0.515 ± 0. 028 ） mg/L and （ 0.518 ± O. 022 ） mg/L, respeetively, while those of PS group were （ 0. 820 ±0. 023 ） mg/L, （ 0. 694 ±0. 019 ） mg/L and （ 0. 579 ± 0. 015 ） mg/L, respectively, which were significan- tly higher in the PS groups （P 〈 0.05 ）. But there were no significant differences between CON group and PS ＋ DI group （ （ 0.599±0. 015 ） mg/L, （ 0.541 ± 0. 0

关 键 词：白介素-1 白介素-6 颞下颌关节 心理应激 

分 类 号：R782.2[医药卫生—口腔医学]