树形分子修饰的碳纳米管颗粒转染胰腺癌细胞BxPC3安全性的研究  

Morphological changes of pancreatic cancer cells transfected by dendrimer modified carbon nanotubes

作　　者：程东峰[1] 吴旭波 王加祥[1] 韩宝三[1] 张雪清[3] 杨浩[3] 袁恒光[3] 朱萍[4] 彭承宏[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院普外科、消化外科研究所,上海200025 [2]上海市闵行区中心医院 [3]上海交通大学微纳米科学技术研究院 [4]上海交通大学医学院电镜教研室

出　　处：《中华胰腺病杂志》2011年第4期269-271,共3页Chinese Journal of Pancreatology

基　　金：上海市科委基金项目（09140902300,10411968500）,国家自然科学基金项目（30772105）

摘　　要：目的观察单壁碳纳米管一聚酰胺树形分子复合物（CNT—PAMAM—D）进人胰腺癌细胞的过程，评价其作为载体的安全性。方法通过超声、搅拌及洗涤等方法制备CNT—PAMAM—D，用原子力显微镜和透射电镜观察其形态。将CNT-PAMAM—D与人胰腺癌细胞BxPC3共同培养12、48、72h，收集细胞，在透射电镜下观察细胞内CNT—PAMAM-D的分布及细胞超微结构的改变。结果PAMAM—D与CNT结合之后，树形分子包裹在CNT的表面，形成了20nm左右大小的纳米复合物颗粒。CNT—PAMAM—D通过细胞吞饮方式被吞入BxPC3细胞，12h后即大量进人细胞质内，随着时间延长，CNT—PAMAM—D还可进入溶酶体及细胞核中，但胰腺癌细胞的形态及超微结构无显著变化。结论CNT-PAMAM—D是一种高效、安全的纳米载体。Objective To observe the process of polyamidoamine dendrimer modified single walled carbon nanotubes complexes （CNT-PAMAM-D） entering into pancreatic cancer BxPC3 ceils, and to evaluate its safety as a vector. Methods The CNT-PAMAM-D were prepared by ultrasound, mixing and rinsing, and the morphology was characterized by atom force microscopy and transmission electron microscope. Then the prepared CNT-PAMAM-D was incubated with human pancreatic cancer cell line BxPC3 cells for 12, 48, 72 hours, and then the cells were collected. Distribution of CNT-PAMAM-D in cells and cell ultrastructure was observed by transmission electron microscopy. Results After the combination of PAMAM-D and CNT, the surface of CNT was surrounded by dendrimer and nanocomposite particles with a size of 20 nm was formed. It was showed that CNT-PAMAM-D could be transfected into human pancreatic cancer cells by cell pinocytotic way, and it entered cytoplasm at 12 h. With the extension of the transfecting time, CNT-PAMAM-D could enter lysosomal and nucleus, but the morphology and uhrastrueture ocf BxPC 3 cells was not significantly changed. Conclusions The CNT-PAMAM-D is a highly effective and safe nano vector.

关 键 词：胰腺肿瘤 碳纳米管 树形分子 透射电子显微镜检查 载体 

分 类 号：R735.9[医药卫生—肿瘤]