急性胰腺炎胰腺腺泡细胞不同死亡方式与细胞内酶释放的关系  

Relationship between different death ways of pancreatic acinar cells and release of intracellular enzymes in acute pancreatitis

作　　者：薛东波[1] 吕明[2] 路广海[1] 张伟辉[1] 潘尚哈[1] 

机构地区：[1]哈尔滨医科大学附属第一医院普外科,哈尔滨150001 [2]美国加利福尼亚大学洛杉矶分校David Geffen医学院外科系

出　　处：《中华胰腺病杂志》2011年第4期281-283,共3页Chinese Journal of Pancreatology

基　　金：黑龙江省教育厅科学技术研究项目（11531157）

摘　　要：目的观察轻重程度不同的体外急性胰腺炎（AP）胰腺腺泡细胞发生凋亡、胀亡的状况及细胞内酶的释放情况，探讨两者的关系。方法以两步酶消化法分离胰腺腺泡细胞，分为4组。AP组加入雨蛙素0．1I．L∥ml，细菌脂多糖（LPS）组加入雨蛙素及LPS（10mg／L），奥曲肽（OCT）组加入雨蛙素及OCT（100n∥fnl），对照组加培养液。应用丫碇橙（ao）和溴乙锭（EB）双染色法检测腺泡细胞的凋亡及胀亡，采用比色法检测培养上清中淀粉酶及乳酸脱氢酶（LDH）含量。结果对照组、AP组、LPS组、OCT组的细胞凋亡指数分别为2．2±0．4、6．4±0．6、4．6±0．4、11，2±1．2；胀亡指数分别为3．0±0．4、17．2±1．6、23．0-t-2．2、12．8±1．4；LDH的分泌量分别为（2180±240）、（8060±930）、（9460±920）、（6860±740）U／dl；淀粉酶的分泌量分别为（1750±190）、（3820zt：460）、（4420±480）、（2260±260）U／L。AP组、LPS组、OCT组的上述4项指标均显著高于对照组（P值均〈0．05）；LPS组的胀亡指数及LDH和淀粉酶分泌量均较AP组显著增加（P值均〈0．05），而凋亡指数则较AP组显著减少（P〈0．05）；OCT组的凋亡指数较AP组显著增多（P〈0．05），而胀亡指数及LDH、淀粉酶分泌均较AP组显著减少（P值均〈0．05）。结论诱导AP胰腺腺泡细胞凋亡，并减少细胞胀亡的发生，可减少腺泡细胞内酶的释放。Objective To observe the apoptosis or oncosis of pancreatic acinar cells of different severity of acute pancreatitis （AP） and the release level of enzymes in vitro, and to investigate the relationship between them. Methods Two-step enzymatic digestion method was used to separate pancreatic acinar cells into 4 groups. 0.1 μg/ml of the caerulein was added in the AP group. Caerulein and LPS （bacterial lipopolysaccharide, 10 rag/L） were added in LPS group. Caerulein and OCT （oetreotide, 100 ng/ml） were added in OCT group. Medium was added in the control group. AO （acridine orange） and EB （ethidium bromide） double staining method was used to detect the incidence of apoptosis or oncosis of acinar cell. The release of intracellular enzyme was detected by measuring the concentrations of amylase and LDH in cell culture media by colorimetry method. Results The apoptosis index was 2.2 ± 0.4, 6.4 ± 0.6, 4.6 ± 0.4, 11.2 ± 1.2 in the control group, AP group, LPS group, OCT group; while the oncosis index was 3.0 ± 0.4, 17.2 ± 1.6, 23.0 ±2.2, 12.8 ±1.4 in the control group, AP group, LPS group, OCT group; the release of LDH was （2180 ±240）, （8060±930）, （9460 ±920）, （6860 ±740）U/dl, the level of amylase was （1750 ± 190）, （3820±460）, （4420±480）, （2260±260）U/L. All the values in the experiment groups were significantly higher than that in control group （ P 〈 0.05 ）. The oncosis index, LDH, amylase in LPS group was significantlyhigher than that in AP group （ P 〈 0.05 ）, but the apoptosis index in LPS group was significantly lower than that in AP group （P 〈 0.05 ）. The apoptosis index in OCT group was significantly higher than that in AP group （P 〈 0.05）, but the oncosis index, LDH, amylase was significantly lower than that in AP group （P 〈 0.05）. Conclusions Induction of apoptosis and reduction of oncosis in AP pancreatic acinar cells can reduce the release of enzyme in acinar ceils.

关 键 词：胰腺炎 细胞死亡 细胞凋亡 淀粉酶类 

分 类 号：R576[医药卫生—消化系统]