Fas基因转染联合应用顺铂对舌鳞癌细胞的杀伤作用  

The killing activity of Fas gene transfection combined with cisplatin on tongue squamous cell carcinoma

作　　者：祖谷[1] 陈乔尔[1] 胡芳芳[1] 方丽[1] 

机构地区：[1]安徽医科大学口腔医学院,合肥230032

出　　处：《安徽医科大学学报》2011年第9期890-892,896,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学研究重点项目(编号:KJ2007A026)

摘　　要：目的探讨Fas基因转染联合应用顺铂对舌鳞癌Tca8113细胞的杀伤作用。方法实验细胞分为未转染Fas基因组和转染Fas基因组,各加入100μl顺铂,使其终浓度分别为1、5、10、20、40μg/ml,观察顺铂作用下转染前、后Tca8113细胞的形态学变化,绘制细胞生长曲线,四甲基偶氮唑蓝(MTT)法检测顺铂对转染前、后Tca8113细胞的抑制率,流式细胞仪测定转染前、后不同浓度顺铂诱导Tca8113细胞的凋亡情况。结果转染Fas基因组可观察到细胞凋亡;转染组Tca8113细胞生长速率低于未转染组;转染组和未转染组Tca8113细胞的增殖抑制作用均随着顺铂浓度的增加而增强;顺铂浓度相同时,转染组Tca8113细胞的增殖抑制作用高于未转染组;未转染Fas基因组顺铂诱导Tca8113细胞,统计分析差异无显著性,而转染组Tca8113细胞的凋亡率随顺铂浓度增加而升高。结论通过Fas基因转染联合应用顺铂,可增加舌鳞癌Tca8113细胞的抑制率,并可提高其对顺铂诱导凋亡的敏感性,顺铂终浓度为20μg/ml时可获得最大细胞凋亡率,同时减少大剂量用药带来的毒性反应。Objective To investigate the effect of Fas gene transfection combined with cisplatin on tongue squamous cell carcinoma cells line Tca8113 in vitro.Methods The cells were divided into two groups： untransfected group and transfected group,we supplemented with cisplatin 100 μl,the final concentration of cisplatin was 1,5,10,20 and 40 μg/ml,respectively,then observed the morphological changes of Tca8113 cells and recorded cell growth curve.The cell inhibiting rate of Tca8113 cells pre-and post-transfection induced by cisplatin were analysed by MTT methods.The apoptosis of tumor cells was detected by flow cytometry（FCM）.Results The apoptosis was observed in transfected group.In both groups,cell inhibiting rate significantly enhanced with the increase of the concentration of cisplatin,but the transfected group was higher than the untransfected group.FCM analysis showed that with the increase of the concentration of cisplatin,cisplatin could not induce more apoptosis could efficiently induce apoptosis in the transfected group.Conclusion Fas gene transfection combined with cisplatin can improve the cell inhibiting rate of Tca8113 and Fas gene transfection can also enhance the killing ability of cisplatin.At the concentration of 20 μg/ml,cisplatin can not only significantly enhance Fas-mediated apoptosis but also reduce the cytotoxicity.

关 键 词：舌肿瘤/治疗 癌 鳞状细胞 转染 细胞凋亡 

分 类 号：R739.86[医药卫生—肿瘤] R394.33[医药卫生—临床医学]