蜂毒素对人肝癌细胞裸鼠移植瘤生长的影响及其部分机制  

Effect and partial mechanisms of melittin on human hepatocellular carcinoma cells xenograft in nude mice

作　　者：王朋景[1] 李俊[1] 黄艳[1] 吴宝明[1] 

机构地区：[1]安徽医科大学药学院,合肥230032

出　　处：《安徽医科大学学报》2011年第9期905-909,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:90413140);高校省级优秀青年人才基金(编号:2009SQR2043);安徽省高校青年教师科研资助项目(编号:2008jq1067)

摘　　要：目的探讨蜂毒素对人肝癌HepG-2细胞株裸鼠移植瘤的抑制作用。方法建立人肝癌HepG-2细胞裸鼠皮下移植瘤模型,随机分为模型组、蜂毒素组(1、2、4 mg/kg)和氟尿嘧啶(5-FU,2 mg/kg)组,各组腹腔注射给药10 d,用药结束后隔天处死动物。观察蜂毒素腹腔注射对人肝癌细胞在裸鼠体内生长的的影响,计算各组裸鼠肿瘤的大小、瘤重及脾指数。ELISA法检测裸鼠血清白介素-1(IL-1)及肿瘤坏死因子-α(TNF-α)。TUNEL法检测裸鼠移植瘤细胞凋亡情况。结果蜂毒素(1、2、4 mg/kg)组的抑瘤率分别为41.2%、60.9%、71.0%,蜂毒素各剂量组肿瘤体积显著小于模型组,而血清IL-1和TNF-α水平显著高于模型组,与模型组比较,蜂毒素(2、4 mg/kg)组肿瘤组织细胞的凋亡指数显著升高(P<0.05,P<0.01)。结论蜂毒素能明显抑制人肝癌细胞HepG-2细胞裸鼠移植瘤的生长,其作用机制可能与增强裸鼠的免疫能力和促进肿瘤组织细胞的凋亡有关。Objective To study the effects of melittin on the growth of human hepatocellular carcinoma HepG-2 cells xenograft in BALB/c nude mice and explore its possible mechanism of antitumor action in vivo.Methods The nude mice with HepG-2 cell xenografts were randomly divided into control group,melittin group（1,2,4 mg/kg）,5-FU group（2 mg/kg）,administered for 10 days.The mice were killed 2 days after treatment.The xenograft tumor growth in mice was measured after injection of melittin.Tumor volume,tumor weight and spleen index were calculated.The levels of interlukin-1（IL-1） and tumor necrosis factor-α（TNF-α） in serum of nude mice were detected by enzyme-linked immunosorbent assay（ELISA）.The apoptosis index of tumor tissue was measured by TUNEL method.Results While injected with 1,2 and 4 mg/kg of melittin,the inhibitory rate of tumor growth were 41.2%,60.9% and 71.0%,respectively.The tumor volume of xenograft in nude mice was significantly smaller in melittin groups than that in control group（P0.01）.Levels of IL-1 and TNF-α,the apoptosis index in the melittin groups were significantly higher than those in the control group（P0.05,P0.01）.Conclusion Melittin could inhibit the growth of tumor in vivo,which might be related to reinforcement of the immunity of mice and the apoptosis of tumor cells.

关 键 词：蜂毒肽 肝肿瘤 实验性 

分 类 号：R735.7[医药卫生—肿瘤] R965.1[医药卫生—临床医学]