磷酸肌酸钠预给药对大鼠局灶性脑缺血再灌注损伤的影响  

Effects of Exogenous Creatine Phosphate Sodium Pre-Conditioning on Rat Model of Focal Cerebral Ischemia/Reperfusion Injury

作　　者：李波[1] 吕国义[1] 孙成亮[1] 邓迺封[1] 

机构地区：[1]天津医科大学第二医院麻醉科,300211

出　　处：《天津医药》2011年第9期838-840,883,共4页Tianjin Medical Journal

摘　　要：目的:观察不同浓度磷酸肌酸钠预给药对大鼠脑缺血再灌注损伤(I/R)模型脑梗死体积的影响。方法:模型建立采用大脑中动脉线栓法。50只SD大鼠随机分为假手术组(Sham组),I/R组及低、中、高剂量磷酸肌酸钠预给药组(即L、M、H组),每组10只。再灌注末,测血清超氧化物歧化酶(SOD)、丙二醛(MDA)水平,脑组织中Na+-K+-ATP酶及髓过氧化物酶(MPO)活性。实验结束对脑组织进行HE、TTC染色,观察脑组织损伤情况。结果:再灌注24h末,各组间血清SOD、MDA及脑组织中MPO、Na+-K+-ATP酶活性比较差异有统计学意义(P<0.05);Bederson神经功能缺损评分显示Sham组无损伤,其余各组均出现不同程度的神经损伤。脑梗死体积百分比比较,Sham组脑组织无梗死,I/R组脑梗死体积百分比较L、M、H组明显增大,L组大于M组、H组;M组脑梗死体积大于H组,差异均有统计学意义(P<0.05)。结论:低、中、高浓度磷酸肌酸钠预给药均可有效地减少局灶性脑缺血再灌注损伤后大鼠脑梗死体积,减轻脑缺血再灌注后的损伤。Objective：To observe the effect of creatine phosphate sodium pre-conditioning on cerebral infarction volume of focal cerebral ischemia/reperfusion rat model.Methods：The rat model of focal cerebral ischemia/reperfusion injury was established by middle cerebral artery occlusion（MCAO） method.Fifty SD rats were randomly divided into 5 groups,sham operation group（Sham）,ischemia/reperfusion injury group（I/R）,low dosage of creatine phosphate sodium group（L）,moderate dosage of creatine phosphate sodium group（M）,and high dosage of creatine phosphate sodium group（H）.The serum levels of superoxide dismutases（SOD） and malondialdehyde（MDA） were measured at the end of reperfusion.The activity levels of Na＋-K＋-ATP enzyme and myeloperoxidase（MPO） were detected in rat brain tissues.Staining methods of hematoxylin eosin（HE） and triphenyltetrazolium chloride（TTC） were used to detect the brain damage at the end of reperfusion.Results：There were significant differences in serum levels of SOD,MDA,and MPO and Na＋-K＋-ATP enzyme in brain tissue between groups at the end of reperfusion（P 0.05）.There was no cerebral infraction in Sham group detected by Bederson score,but there were different degrees of injury in other groups.The volume of cerebral infraction was significantly larger in I/R group compared with that of L,M and H groups（P 0.05）,no cerebral infraction was found in Sham group.The volume of cerebral infraction was significantly larger in L group than that of M and H groups（P 0.05）.The volume of cerebral infraction was significantly larger in M group than that of H group（P 0.05）.Conclusion：Pre-conditioning of creatine phosphate sodium can attenuate ischemia/reperfusion injury in rat model of focal cerebral ischemia.

关 键 词：磷酸肌酸 钠 脑梗死 再灌注损伤 超氧化物歧化酶 丙二醛 过氧化物酶 钠钾交换ATP酶 大鼠 Sprague-Dawley 

分 类 号：R743.31[医药卫生—神经病学与精神病学]