转化生长因子β1注射退变椎间盘内软骨终板的组织形态变化  

Histomorphological changes of cartilage endplate of degenerated intervertebral discs treated by transforming growth factor beta 1

作　　者：杨学军 马骁 霍洪军 房芳 

机构地区：[1]Department of Spine Surgery, Second Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010030, Inner Mongolia Autonomous Region, China [2]Department of Orthopedics, the 253 Hospital of Chinese PLA, Hohhot 010051, Inner Mongolia Autonomous Region, China [3]Postgraduate School, Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China

出　　处：《中国组织工程研究与临床康复》2011年第28期5149-5152,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：内蒙古自治区自然科学基金资助项目(200508010908)资助,项目名称:椎间失稳导致椎间盘退变的机理及阻止和逆转的实验研究~~

摘　　要：背景:椎间失稳能导致软骨终板的退变,是椎间盘退变发病机制中的基础环节。目的:观察退变椎间盘内注射转化生长因子β1后,椎间盘软骨终板的组织形态学变化。方法:将日本大耳白兔随机分为对照组、预防组和治疗组。所有兔均建立L5～6,L6～7椎间失稳模型。预防组在完成椎间失稳建模后立即于损伤侧L5～6,L6～7椎间盘内注射转化生长因子β1,治疗组于椎间失稳建模后3个月行相同方法注射转化生长因子β1。结果与结论:建模后3,6个月,预防组较对照组软骨终板软骨细胞分布均匀,潮线清晰。Mankin评分降低(P<0.05)。建模后第6个月治疗组较对照组软骨终板表层光滑,细胞排列均匀,潮线清晰,染色均匀,Mankin评分降低(P<0.05)。结果证实,在兔椎间盘内注射转化生长因子β1可延缓椎间盘软骨终板的退变。BACKGROUND：The intervertebral destabilization can distinctly lead to the degeneration of the cartilage endplate. Suitable concentration of transforming growth factor beta 1（TGF-β1） can stimulate proliferation, division and differentiation of articular chondrocytes. OBJECTIVE：To observe the histomorphological change of cartilage endplate of degenerated intervertebral discs treated by TGF-β1. METHODS：Forty-six white Japanese rabbits, weighing 2.5±0.2 kg, were randomly divided into three groups：control group （n=18）, preventive group （n=18） and treatment group （n=10）. L5-6, L6-7 intervertebral destabilization was created in all rabbits. The preventive group rabbits were injected TGF-β1 into the L5-6 and L6-7 intervertebral disc on the injection side immediately after model establishment. The treatment group rabbits received the same injection at 3 months after model establishment. RESULTS AND CONCLUSION：At 3 and 6 months after model establishment, compared with the control group, chondrocytes were more evenly distributed in the cartilage endplate with clear tidal line and Mankin score was decreased in the preventive group （P 0.05）. At 6 months after model establishment, compared with control group, cartilage endplate surface was smooth, cells were orderly arranged, tidal line was clear, hematoxylin-eosin staining was more even, and Mankin score was reduced in the treatment group （P 0.05）. These findings suggest that TGF-β1 can prevent and treat degeneration of the cartilage endplate due to the intercertebral destabilization.

关 键 词：转化生长因子 椎间失稳 软骨终板 组织形态学 退变 椎间盘 骨组织工程 

分 类 号：R575.2[医药卫生—消化系统]