重组腺病毒Ad-ING4和Ad-PTEN抑制肝癌细胞增殖  

Investigation of inhibition of adenovirus mediated gene ING4 and PTEN to hepatocarcinoma cells

作　　者：姚雷[1] 曹婧媛[1] 吴鹏飞[1] 黄玉洪[1] 王金志[2] 杨吉成[2] 缪竞诚[2] 

机构地区：[1]苏州大学医学部附属第一医院,江苏苏州215000 [2]苏州大学医学部细胞与分子生物教研室,江苏苏州215000

出　　处：《基础医学与临床》2011年第9期976-979,共4页Basic and Clinical Medicine

基　　金：国家级大学生创新性实验计划项目(SG315859)

摘　　要：目的用构建好的重组腺病毒Ad-ING4和Ad-PTEN感染人肝癌SMMC-7721细胞,检验其对细胞的作用。方法用构建好的重组腺病毒Ad-ING4和Ad-PTEN感染QBI-293A细胞,对病毒进行扩增并测定其效价,荧光显微镜下观察感染效果。用扩增得到的Ad-ING4和Ad-PTEN感染SMMC-7721细胞,流式细胞术及MTT检测作用效果。结果空腺病毒组的凋亡率为3.4%±1.3%,Ad-ING4组和Ad-PTEN组分别为49.7%±4.8%和11.4%±3.2%,均显著高于空腺病毒组（P〈0.01）;Ad-ING4组和Ad-PTEN组的细胞增殖在48~96 h时较正常组明显下降（P〈0.05）。结论 Ad-ING4和Ad-PTEN对SMMC-7721细胞均有显著抑制作用,尤其是Ad-ING4。Objective We use recombinant Ad-ING4 and Ad-PTEN to infect human hepatocarcinoma cell line SMMC-7721 in vitro to test and compare the effects of gene expression of the cells.Methods We use recombinant Ad-ING4 and Ad-PTEN and control virus to infect QBI-293A cells for viral propagation and viral titration.The infective effects of Ad-ING4,Ad-PTEN and control virus were confirmed by fluorescence microscopy.Use Ad-ING4 and Ad-PTEN to infect SMMC-7721 cells and confirm their effects by their apoptotic rate and the results of MTT.Results The apoptotic rate of control virus group is 3.4%±1.3%,the Ad-ING4 group is 49.7%±4.8%（P〈0.01）and the Ad-PTEN group is 11.4%±3.2%（P〈0.01）.The cell proliferation of group Ad-ING4 and Ad-PTEN obviously decreased from 48h to 96h（P〈0.05）.Conclusion The data analysis showed Ad-ING4 and Ad-PTEN had an inhibitory effect to SMMC-7721 cells especially that of Ad-ING4.

关 键 词：肝癌 AD-ING4 Ad-PTEN 基因 

分 类 号：R735.7[医药卫生—肿瘤]