大鼠实验性蛛网膜下腔出血后脑内HSP72表达增加  

Overexpression of HSP72 in cortex and hippocampus after experimental subarachnoid hemorrhage in rats

作　　者：范议方[1] 韩如泉[1] 

机构地区：[1]首都医科大学附属北京天坛医院麻醉科,北京100050

出　　处：《基础医学与临床》2011年第9期980-983,共4页Basic and Clinical Medicine

基　　金：北京市科委科技新星资助项目(2007B74);教育部留学回国人员科研启动基金

摘　　要：目的研究大鼠蛛网膜下腔出血后HSP72在大脑皮质和海马区的表达。方法 36只Wistar大鼠随机分为假手术组(SO)、枕大池1次注血组(SI)及2次注血组(DI)分别制作蛛网膜下腔出血模型。制备模型成功后取脑行免疫组化染色及Western blot检测海马和大脑皮质区HSP72的表达。结果海马及大脑皮质有HSP72的表达;与假手术组相比,注血组海马及大脑皮质区HSP72的表达显著增加(SI:0.63±0.17,0.62±0.25;DI:0.81±0.25,0.83±0.34;P<0.05)。结论蛛网膜下腔出血可造成大鼠早期脑损伤,并引起海马及脑皮质HSP72的过度表达。Objective To assess the expression of HSP72 in cortex and hippocampus after experimental subarachnoid hemorrhage in rats.Methods Thirty-six wistar rats were randomly divided into three groups： sham operation,single intracisternal blood injection and double intracisternal blood injection.The expression of HSP72 in hippocampus and cerebral cortex was detected by immunohistochemistry and Westernblot technique.Results HSP72 expression was detected in intracisternal blood injection group by immunohistochemistry.Semiquantitative analysis of HSP72 electrophoretic bands indicated more expression in hippocampus and cerebral cortex in both injection groups compared with the sham group（SI： 0.63±0.17,0.62±0.25;DI： 0.81±0.25,0.83±0.34;P〈0.05）.Conclusion Subarachnoid hemorrhage produced by single and double intracisternal autogenious blood injection can cause HSP72 overexpression in hippocampus and cerebral cortex.

关 键 词：蛛网膜下腔出血 脑血管痉挛 HSP72 

分 类 号：R743.3[医药卫生—神经病学与精神病学]