肾癌中MIR-184的异常表达及意义  

Abnormal expression and significance of MIR-184 in human renal carcinoma

作　　者：冷慧敏[1,2] 钱卫平[1] 周亮[1] 翟庆娜[2] 李贤新[3] 关志忱[3] 桂耀庭[4] 蔡志明[4] 

机构地区：[1]北京大学深圳医院生殖医学科,深圳518036 [2]汕头大学医学院,汕头5150313 [3]北京大学深圳医院泌尿外科,深圳518036 [4]北京大学深圳医院男性生殖实验室,深圳518036

出　　处：《北京大学学报（医学版）》2011年第4期509-513,共5页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(30900817);深港创新圈计划(ZYB200907080110A);深圳市科技研发资金基础研究计划资助项目(JC201005260208A);深圳市生物;互联网;新能源产业发展专项资金(CXC201005260001A)资助~~

摘　　要：目的:探讨microRNA-184(MIR-184)在肾细胞癌发生过程中的作用。方法:应用实时荧光定量PCR(RT-qPCR)技术检测MIR-184在51例不同阶段肾癌及其癌旁组织中的相对表达量,并分析其表达量与肾癌患者年龄、性别及临床分期的相关性。结果:MIR-184在肾癌组织的平均表达量为-14.664 6±5.362 4,在相应癌旁组织中的平均表达量为-10.408 7±3.482 7,MIR-184在肾癌与癌旁组织中的表达差异具有统计学意义(P<0.01)。以MIR-184在肾癌组织中表达量的平均值-14.664 6为界,将患者分为MIR-184低表达组和MIR-184高表达组,同时分别按照年龄、性别、临床分期将患者分为3组。卡方检验统计分析结果显示:癌组织中MIR-184的表达高低与患者的年龄、性别及肾癌的临床分期均没有相关性(具体概率分别为:P>0.30,P>0.99,P>0.30)。结论:MIR-184在肾癌发生、发展过程中发挥着重要的作用。Objective： To explore the role of microRNA-18 4（MIR-184） in the development of renal cell carcinoma（RCC）.Methods： The expressions of MIR-184 in 51 patients with RCC Investigated,normal adjacent tissues（ADTs） matched by fluorescence quantitative PCR technology（RT-qPCR） and the correlations analyzed between MIR-184 expression and the age,gender and clinical stage of RCC patients.Results： The average expression of MIR-184 in RCC was-14.664 6±5.362 4,while that in ADTs was-10.408 7±3.482 7（P〈0.01）.Bounded with the MIR-184 expression in RCC,patients were divided into lower-expression group and higher expression group.Meanwhile,the RCC patients were divided into three groups according to the age,gender and clinical stage of the patients.Chi-square statistical analysis showed that the expression level of MIR-184 was not significantly correlated with the patient＇s age,gender and clinical stage（respectively：P〉0.03,P〉0.99,P〉0.03）.Conclusion： MIR-184 in RCC was significantly lower than that in ADTs,which may have potential significance in the occurrence and development of RCC.

关 键 词：微RNA 癌 肾细胞 肿瘤分期 

分 类 号：R737.11[医药卫生—肿瘤]