曲古抑菌素A对舌鳞癌SCC-6细胞生物学行为的影响  

Effects of TSA on Cell Growth,Cell Cycle,Apoptosis and Invasion of Tongue Squamous Cell Carcinoma SCC-6 Cell Line in vitro

作　　者：吴湣[1] 康非吾[1] 阙林[1] 王开[1] 

机构地区：[1]同济大学附属口腔医院口腔颌面外科,上海200092

出　　处：《口腔颌面外科杂志》2011年第4期242-246,共5页Journal of Oral and Maxillofacial Surgery

基　　金：上海市科学技术委员会青年科技启明星计划项目(09QA1406400)

摘　　要：目的:观察曲古抑菌素A(TSA)对舌鳞癌细胞生物学行为的影响,探讨其体外抗癌活性。方法:不同浓度、时间梯度TSA处理人舌鳞癌SCC-6细胞后,MTT法检测细胞增殖活性;流式细胞仪定量检测细胞周期和凋亡变化;Transwell法测定细胞体外侵袭性改变。结果:①TSA对SCC-6细胞的增殖有明显抑制作用,并呈剂量和时间依赖性;②随着TSA浓度的增加,SCC-6细胞G1期的细胞数目逐渐减少,而S期和G2/M期的细胞数目增加,提示TSA将细胞周期抑制在S期和G2/M期,同时随着TSA浓度的增加,SCC-6细胞的凋亡率与对照组相比明显增加(P<0.05),提示TSA能促进SCC-6细胞的凋亡;③经不同浓度TSA处理24 h后,SCC-6细胞穿透人工基质膜的细胞数随着TSA浓度上升而逐渐减小,提示TSA能显著抑制SCC-6细胞的侵袭性。结论:体外条件下,TSA能明显抑制SCC-6细胞的增殖和侵袭能力,诱导其凋亡。而其所介导的细胞凋亡很可能与S期和G2/M期细胞周期的阻滞有关。Objective： This study was aimed to investigate weather trichostatin A can affect cell growth, apoptosis, cell cycle, and invasiveness of tongue squamous cell carcinoma SCC-6 cell line in vitro, which reveals the anticancer activities. Methods： SCC-6 cells were treated with TSA in different concentrations with various time intervals in vitro. Proliferative activities of these cells were assessed by MTT assay. Cell cycle and cell apoptosis were analyzed by flow cytometry using Annexin V, and invasive properties were detected by Transwell ways. Results： TSA inhibited the proliferation of SCC-6 cells in a dose- and time-dependent manner. TSA arrested the cell cycle in G2/M phase with the decreasing amount of G2 phase and the increasing of S and G2/M phase. The apoptosis rate increased gradually in dose- and time-dependent manner, which showed significant difference comparing with control group （P〈0.05）. TSA inhibited the invasion rate of SCC-6 cells at different concentrations （P〈0.05）. Conclusion： In vitro, TSA shows potential on anticancer activities, inhibits SCC-6 cells proliferation and invasion, arrests the cell cycle in G2/M phase which induces cell apoptosis.

关 键 词：曲古抑菌素A 舌鳞癌 增殖 细胞周期 凋亡 侵袭性 体外实验 

分 类 号：R739.86[医药卫生—肿瘤] R329.28[医药卫生—临床医学]