靶向性超声显影评价不同程度肝损伤的初步实验研究  

Preliminary experimental study on evaluation of liver injury by ultrasonic targeting imaging

作　　者：余进洪[1] 郑元义[1] 李奥[1] 王志刚[1] 项莹霞[2] 

机构地区：[1]重庆医科大学附属第二医院超声科,重庆市400010 [2]重庆医科大学附属第二医院消化科,重庆市400010

出　　处：《临床超声医学杂志》2011年第8期505-508,共4页Journal of Clinical Ultrasound in Medicine

基　　金：国家青年科学基金项目(30900370)

摘　　要：目的使用靶向超声分子显像对比分析大鼠不同程度急性肝损伤后峰值回声强度的改变与去唾液酸糖蛋白受体(ASGPR)的含量及血清学检查的关系。方法根据腹腔灌注四氯化碳分析纯的不同剂量(10%、20%、30%、40%、50%),将45只健康雄性SD大鼠随机分为相应5组(组Ⅰ～组Ⅴ),每组9只,每组中再随机抽取4只取肝组织,分别检测ASGPR的含量;另5只仅常规麻醉,胸腹部备皮,经尾静脉团注靶向液态氟碳纳米脂质超声造影剂。从注射开始至0.5h内连续观察并记录,0.5～1h内每10min观察并记录,1h至造影剂消退每30min观察并记录肝实质超声图像。DFY型超声图像定量分析诊断仪对各时间点采集到的超声图像进行分析。另选10只健康雄性SD大鼠作为靶向超声造影对照组,方法同前。结果随着肝损伤程度的增加,肝组织内的ASGPR含量逐渐减少,且各组样本均数两两比较差异均有统计学意义(P<0.05)。同时肝靶向超声造影后峰值回声强度逐渐降低,除对照组与组Ⅰ比较差异无统计学意义外,其余各组两两比较差异均有统计学意义(P<0.05),而达峰时间、峰值回声强度、造影前肝实质回声强度及清退后肝实质回声强度各组间比较差异均无统计学意义(P>0.05)。结论自制靶向肝实质细胞膜受体ASGPR的液态氟碳纳米超声造影剂的肝靶向性显影效果佳;不同程度肝损伤后的靶向超声显影结果改变与ASGPR结果改变一致,有利于超声显影定量评价肝脏功能。Objective To comparatively analyze the relationship of peak - echo intensity of targeted ultrasound molecular imaging and asialoglycoprotein receptor （ ASGPR ） content in different degree of rat acute liver injury. Methods Acute liver injury model was established by intraperitoneal infusion of carbon tetrachloride （ CC]4 ） in 45 healthy male SD rats, the rats were divided into 5 groups （ group I - group V ） according to doses of CCI4 （ 10% ,20% ,30% ,40% ,50% ） with 9 rats in each group. The content of liver ASGPR in 4 rats of each group was detected respectively. The others were anesthetized generally, thoracic and abdominal skin was prepared, the liquid perfluorocarbon nanoparticles targeted ultrasound contrast agents were injected through the tail vein with bolus injection. From the beginning to 0.5 h, the ultrasonic imaging was observed and recorded continuously; from 0.5 h to 1 h, the ultrasonic imaging was observed and recorded in each 10 rain; from 1 h to the regression of contrast agents, the ultrasonic imaging of the liver parenchyma was observed and recorded in each 30 min. Finally, the ultrasonic images of the liver parenehyma at different time points were analyzed by DFY diagnostic ultrasound image analysis system. Another 10 healthy male SD rats were used as control group. Results With the increase of liver damage, the ASGPR content of liver tissue was decreased, there was significant difference between the groups（P 〈 0.05 ） ; with the increase of injury degree, the peak echo intensity of liver was decreased, there was significant difference between the groups （ P 〈 0.05 ） , except the normal control group and group ] （P 〉 0. 05 ）. While there was no significant difference of time to peak, peak intensity, parenchyma intensity before enhancement and parenchyma intensity after wash - out of contrast agents （P 〉 0.05）. Conclusion The imaging of liver injury with liquid perfluorocarbon nanoparticles ultrasound contrast agents targeted to liver cell membrane recepto

关 键 词：超声分子显像 达峰时间 峰值回声强度 肝脏储备功能 

分 类 号：R575[医药卫生—消化系统] R445.1[医药卫生—内科学]