大鼠实验研究中三种采血方法的应用  

Application of Three Rat Blood Collection Methods in Experiments

作　　者：邹洪[1,2] 闫洪涛[1] 李军[3] 伍松[1] 唐均成[1] 汤礼军[1] 

机构地区：[1]成都军区总医院普外中心,610083 [2]泸州医学院 [3]成都军区总医院普麻醉科,610083

出　　处：《西南军医》2011年第5期787-788,共2页Journal of Military Surgeon in Southwest China

摘　　要：目的探讨大鼠眼眶后静脉丛真空采血管连续采血法的可行性。方法将60只GK大鼠随机分为三组(每组20只),分别使用三种不同采血法,比较它们的成功率。眼眶后静脉丛的采血法:垂直插入内眦并向眼底方向转动以便切开静脉丛。腹主动脉采血法:在腹主动脉分叉处向心端1～3 mm处为最佳穿刺点。尾尖采血方法:手术剪剪去尾尖5～10 mm,三种方法各采血80次。结果成功率分别为:眼眶后静脉丛采血100%;腹主动脉采血95%;尾部采血100%。结论眼眶后静脉丛真空采血管采血法具有取血量大,不易溶血,不损伤器官且可反复多次采集等特点,有利于提高实验的准确性。Objective To discuss the feasibility of incessant blood collection with vacuum tube in post-orbital venous plexus of rats.Methods 60 GK rats were randomly divided into 3 groups,20 in each,and 3 different blood collection methods were applied respectively in the 3 groups： blood collection from post-orbital venous plexus,blood collection from abdominal aorta and blood collection from the tail tip;each method was performed for 80 time and their successful rates were compared.Results The successful rate of blood collection from post-orbital venous plexus was 100% as well as blood collection from tail tip,the successful rate of blood collection from abdominal aorta was 95%.Conclusions Blood collection with vacuum tube in post-orbital venous plexus is of such advantages as： bigger blood collecting volume,lower occurrence of hemolysis,no wound to organs and repeatability of collection,it can raise the accuracy of experiments.

关 键 词：眼眶后静脉丛 真空采血管 采血法 

分 类 号：R-332[医药卫生]