癫痫持续状态后神经原损伤与耐药基因产物P-gp关联性研究  

Association of neuronal injury following status epilepsy and expression of P-glycoprotein

作　　者：王立威[1] 谭东[2] 高明[3] 曹小帅[3] 刘松岩[3] 

机构地区：[1]长春市人民医院,吉林长春130051 [2]长春市第二医院,吉林长春130062 [3]吉林大学中日联谊医院,吉林长春130033

出　　处：《中国实验诊断学》2011年第8期1257-1258,共2页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省自然科学基金项目(NO.200705268);长春市科技计划项目(NO.200808608SF14)

摘　　要：目的研究癫痫发作后神经元损伤与耐药基因产物P-gp的关系,探讨难治性癫痫与神经元损伤的关联性。方法采用氯化锂-匹罗卡品法制备癫痫持续状态大鼠动物模型,随机分为健康对照组、癫痫持续状态(SE)后6h、12h、1d、3d、5d及7d组。采用图像分析法进行神经原损伤的病理学研究;流式细胞仪检测血液中P-gp表达阳性细胞率;应用Pearson相关性统计法得出癫痫发作后神经原损伤与P-gp表达的相关性。结果癫痫持续状态导致神经原损伤,3-7天最明显;相应时间点P-gp表达也同步增高;神经原损伤严重程度与P-gp表达呈正相关。结论难治性癫痫与癫痫发作后神经原损伤相关。Objective To study the association of neuronal injury following status epilepsy and expression of P-glycoprotein,discuss the association of intractable epilepsy and neuronal injury.Methods To establish a mice status epilepticus model by lithium-pilocarpine injected intraperitoneal,The mice were randomly divided into control group,6 h、12 h、1 d、3 d、5 d and 7 d group after status epilepticus（SE）.To research the neuronal injury by image analysis method,expression of P-gp in blood was studied by flow cyometry.To analysis the association between neuronal injury and expression of P-gp by Pearson statistics.Results The neuronal injury was observed following status epilepsy,neuronal injury is serious at 3-7day after status epilepsy,expression of P-gp synchronized increased,the severity of neuronal injury has a positive correlation with expression of Pgp.Conclusion There is association between the intractable epilepsy and neuronal injury.

关 键 词：神经元损伤 P-糖蛋白 难治性癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]