塞来昔布对人甲状腺髓样癌TT细胞增殖及细胞周期分布的影响  

Effect of celecoxib on proliferation of TT cell in human thyroid medullary carcinoma

作　　者：孟宪瑛[1] 王广义[1] 张强[1] 华特波[1] 任江[1] 刘嘉[1] 

机构地区：[1]吉林大学白求恩第一医院甲状腺外科,吉林长春130021

出　　处：《中国实验诊断学》2011年第8期1259-1261,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省卫生厅资助项目(编号2008Z017)

摘　　要：目的探讨塞来昔布对甲状腺髓样癌TT细胞体外生长及细胞周期分布的影响。方法采用3H-TdR掺入法比较不同浓度的塞来昔布对TT细胞增殖的抑制效应,流式细胞术检测肿瘤细胞周期分布的变化情况。结果 3H-TdR掺入法显示不同浓度的塞来昔布均对肿瘤细胞的增殖有明显抑制作用,并在80μmol/L以下时呈浓度依赖性(F=93.83,P<0.05),随时间的延长及浓度高于80μmol/L时虽然CPM值有下降的趋势,但无统计学意义(P>0.05)。流式细胞仪检测发现塞来昔布使TT细胞周期发生了G0/G1期阻滞,并成浓度、时间依赖性,G2期与S期细胞数均较用药前减少,G2期无显著差异(P>0.05),S期有显著差异(P<0.05)。结论塞来昔布可通过抑制环氧合酶-2活性,抑制甲状腺髓样癌TT细胞的增殖,阻止细胞周期进展,明显降低其增殖指数,在诱导其凋亡中起重要作用,COX-2可作为甲状腺癌治疗的新靶点。Objective To disscuss the effect of celecoxib on TT cell vitro growth and cell cycle distribution of thyroid medullary carcinoma.Methods Compared the depressive effect of celecoxib in different density on TT cell proliferation by 3H-TdR incorporation method,the disposition of tumor cell cycle was detected by flow cytometry.Results 3H-TdR incorporation method showed that celecoxib of any density had obvious depressant effect on tumor cell proliferation and manifested concentration dependent when the density was under 80 μmol/L（F=93.83,P0.05）.CPM decreased with the extension of time and the density was above 80 μmol/L,but it had no statistically significant（P0.05）.Flow cytometry manifested TT cell cycle block was happened in G0/G1 stage with time and density dependence,cell population was decreased in G2 and S stage with statistically significant in S stage（P0.05） and no statistically significant in G2 stage（P0.05）.Conclusion Celecoxib can inhibit TT cell proliferation and cell cycle by depressing COX-2＇s activity,the proliferation index is decreased obviousy,which plays an important effect on apoptosis induction.COX-2 can be treated as an new therapic target in thyroid carcinoma.

关 键 词：塞来昔布 甲状腺肿瘤 细胞增殖 细胞周期 

分 类 号：R736.1[医药卫生—肿瘤]