一效膏促进小鼠皮肤溃疡愈合的实验研究  

A Effect Creams Promote Human Skin Ulcer Healing of Experiments

作　　者：宋珊珊[1] 李大勇[2] 吕延伟 

机构地区：[1]辽宁中医药大学第一临床学院,辽宁沈阳110032 [2]辽宁中医药大学附属医院血管外科,辽宁沈阳110032

出　　处：《辽宁中医药大学学报》2011年第9期40-42,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金："十一五"国家科技支撑计划项目(2008BAI53B012)

摘　　要：目的:通过观察一效膏对小鼠皮肤溃疡动物模型的疗效影响及比较研究,探讨一效膏对皮肤溃疡的治疗机制,为临床上运用一效膏治疗皮肤溃疡提供实验依据。方法:54只清洁级小鼠随机分为实验组、阳性对照组及模型对照组,建立皮肤溃疡模型,对实验组皮损部位外敷一效膏,阳性对照组皮损部位外敷贝复济,模型对照组皮损部位外敷生理盐水。定期肉眼观察溃疡、测量面积并计算收缩率;做常规病理、VEGF、EGFR和CD34免疫组化方法标记观察毛细血管表达情况并进行计算。结果:治疗过程中,脱落小鼠4只,造模后3、7、12天实验组与阳性对照组创面收缩率好于模型对照组(P<0.05);造模后12天常规病理显示3组间有差异,免疫组化VEGF、EGFR和CD34标记显示,实验组和阳性对照组之间无显著差异,但两组均与模型对照组之间有显著差异(P<0.05)。结论:一效膏可促进小鼠皮肤溃疡的愈合,改善皮肤溃疡局部血液循环,促进成纤维细胞增殖和新生毛细血管形成、肉芽组织生长。Objective：Through the observation of Yixiao ointment of animal models of curative effect,this paper discusses the influence and a comparative study of Yixiao ointment,treatment function and mechanism for clinical use Yixiao ointment to provide the evidence that the treatment of skin ulcers.Method：54 only clean level randomly divided into experimental mice,positive control group and control group,established model skin ulcers model for vitiligo patches on Yixiao ointment,positive effect creams vitiligo patches on the external BeiFu-ji,model control group vitiligo patches on the external saline.Regular macroscopic observation ulcer and measurement area,calculation of shrinkage rate;Do regular pathology,VEGF,EGFR and immunohistochemical method CD34 expression and mark observation capillary is calculated.Results： The course of treatment,fall off mice,made only four the 3d,7d,12d group and control group wounds in positive shrinkage model control group（P〈0.05）.Made 12d conventional pathology revealed after modulus have difference between groups of three,immunohistochemical VEGF,EGFR and CD34 markers showed the group and control group had no significant differences between positive,but two groups are associated with significant differences between model control group（P〈0.05）.Conclusion：Yixiao ointment can promote the healing of mice,improve skin ulcers skin ulcers local blood circulation,promote fibroblasts proliferation and newborn capillary formation,granulation tissue growth.

关 键 词：一效膏 皮肤溃疡 实验研究 

分 类 号：R222.19[医药卫生—中医基础理论]