肺癌与人微小RNA miR-499-3p种子序列遗传变异关系  

Genetic variant in seed region of hsa-miR-499-3p(rs3746444 A>G) increases risk of lung cancer

作　　者：凌晓璇[1,2,3] 黎银燕[1] 杨磊[1] 纪卫东[1] 宾晓农[1] 吕嘉春[1] 

机构地区：[1]广州医学院化学致癌研究所呼吸疾病国家重点实验室,广东广州510182 [2]广州医学院公共卫生学院流行病学与卫生统计学教研室 [3]广东医学院公共卫生实验教学中心

出　　处：《中国公共卫生》2011年第9期1105-1107,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30872142;30872178;81072366);广东省自然科学基金(7003060;0711550;070060;8251018201000005);广东省高等学校高层次人才项目(2010-79)

摘　　要：目的探讨人微小RNA hsa-miR-499-3p种子序列1个常见单核苷酸多态位点（rs3746444 A〉G）的遗传变异与中国南方人群肺癌发病的关联。方法以病例-对照研究方法收集原发性肺癌患者526例及正常对照526人,采用聚合酶链反应-限制性片段长度多态（PCR-RFLP）技术检测hsa-miR-499-3p种子序列的单核苷酸多态（rs3746444 A〉G）的基因型,用SAS 9.13软件进行非条件Logistic回归,校正混杂因素影响,分析基因变异与肺癌发病的关联。结果以携带野生型纯合子rs3746444AA基因型为参照,携带AG基因型个体发生肺癌的危险度可增加32%（校正OR=1.32,95%CI=1.01-1.84）,携带GG基因型的个体患肺癌的危险度增加122%（OR=2.22,95%CI=1.52-3.23）,且rs3746444 G变异的等位基因个数与肺癌发病危险呈剂量-效应关系（趋势性检验P=3.04×10-5）。结论 hsa-miR-499-3p种子序列（rs3746444 A〉G）遗传变异可增加人群肺癌发病的危险性。Objective To explore the association between single nucleotide polymorphism（SNP） in the seed region of hsa-miR-499-3p（rs3746444 A 〉 G） and lung cancer risk among southern Chinese population. Methods In a case-control study,we recruited 526 lung cancer cases and 526 age and sex frequency-matched cancer-free controls. PCR-resriction fragment length polymorphrism（RFLP） was used to genotype rs3746444 and unconditional logistic regression was conducted to analyze the association between the SNP and lung cancer risk with SAS 9.13. Results Compared with the AA genotype, AG heterozygote marginally increased the risk of lung cancer（ adjusted odds ratios[ OR] = 1.32,95% confidence in terval [ CI] ：1.01 - 1.81 ; P 〈0. 05） ,while the GG homozygote correlated with a 1.22-folds increased risk of lung cancer（95% CI： 1.52 - 3.23; P 〈 0. 0001 ）. There was a significant trend for an allele dose-effect on risk of lung cancer （P-trend 〈 0. 0001 ）. Conclusion The genetic variant in the seed region of hsa-miR-499-3p （rs3746444 A 〉 G） could increase the risk of lung cancer.

关 键 词：肺癌 MICRORNA 单核苷酸多态 

分 类 号：R734.2[医药卫生—肿瘤]