咖啡因联合辐射处理对人乳腺癌易感基因BRCA1表达的影响  

Effects of Caffeine Cotreatment With Radiation on Breast Cancer Susceptibility Gene BRCA1

作　　者：李宁[1,2,3,4] 张红[1,2,3] 王燕玲[1,2,3] 周鑫[1,2,3,4] 郝冀方[1,2,3] 

机构地区：[1]中国科学院近代物理研究所,甘肃兰州730000 [2]中国科学院重离子束辐射生物医学重点实验室,甘肃兰州730000 [3]甘肃省重离子束辐射医学应用基础重点实验室,甘肃兰州730000 [4]中国科学院研究生院,北京100049

出　　处：《原子能科学技术》2011年第1期124-128,共5页Atomic Energy Science and Technology

基　　金：国家自然科学基金资助项目(10835011;10805064);甘肃省中青年科技基金资助项目(099RJYA015)

摘　　要：研究了咖啡因对碳离子束辐射的增敏效应,并观察在该过程中人乳腺癌易感基因BRCA1表达的变化。碳离子束联合咖啡因处理人乳腺癌细胞MCF-7后,利用实时细胞检测仪观察细胞生长、流式细胞术检测细胞周期分布变化、real-time PCR检测BRCA1mRNA水平、Western blot检测BRCA1蛋白水平及其磷酸化水平。结果显示:咖啡因处理导致细胞生长明显受到抑制;咖啡因废除了辐射诱导的G2期阻滞,抑制了BRCA1mRNA和蛋白表达水平,同时特异性抑制了BRCA1的丝氨酸1524位点的磷酸化作用。这些结果表明咖啡因抑制了BRCA1蛋白和其磷酸化表达水平。The sensitizing effect of caffeine to carbon ion radiation was investigated and the change of BRCA1 expression was observed.The MCF-7 breast carcinoma cells were exposed to carbon ion beams with or without caffeine.The cell survival was automatically monitored by RT-CES system.Cell cycle distribution was assessed by flow cytometry.The levels of BRCA1 mRNA were analyzed by real-time RT-PCR.The expression of BRCA1 protein and its phosphorylation were examined by Western blot.The results show that caffeine increases the sensitivity of MCF-7 cells to carbon ion radiation,and abrogates the radiation-induced G2 arrest.Caffeine inhibits radiation-induced BRCA1 expression both at mRNA and protein level.At the same time,caffeine specifically abolishes BRCA1 phosphorylation of Ser-1524.The data implicate that caffeine inhibits the expression of BRCA1 protein and its phosphorylation.

关 键 词：BRCA1 离子辐射 咖啡因 辐射敏感性 G2期检测点 

分 类 号：Q247[生物学—细胞生物学]