磷素对不同大豆品种籽粒异黄酮含量的影响  

Effect of Phosphorus on the Content of Kernal Isoflavone in Different Soybean Varieties

作　　者：张勇[1,2] 接伟光[2] 蔡柏岩[1,2] 白莉[2] 王丽阳[1] 李二平[3] 

机构地区：[1]黑龙江大学生命科学学院分子生物学重点实验室,哈尔滨150080 [2]黑龙江东方学院,哈尔滨150086 [3]东北林业大学,哈尔滨150040

出　　处：《中国农学通报》2011年第21期68-72,共5页Chinese Agricultural Science Bulletin

基　　金：黑龙江省教育厅科学技术研究(指导)项目"磷素对不同大豆品种生理活性物质含量的影响"(11553079);黑龙江省教育厅科学技术研究(指导)项目"AM真菌与重迎茬大豆产质量关系的研究"(11553080);黑龙江大学高层次人才支持计划项目(生态修复团队)(Hdtd2010-12);黑龙江省自然科学基金资助项目"磷有效性对大豆菌根侵染的调控及其与磷素代谢和产质量关系的研究"(C200918);黑龙江省博士后科研启动基金项目"磷有效性对大豆菌根侵染的调控及其与磷素代谢和产质量关系的研究"(LBH-Q09022)

摘　　要：为探索磷素对不同大豆品种籽粒异黄酮含量的影响,寻找不同基因型大豆品种最佳施磷水平,以期提高大豆籽粒异黄酮的含量,改善其品质。选用‘黑农48’(高蛋白品种)、‘黑农37’(中间型品种)、‘黑农44’(高油品种)3个大豆品种作为试验材料。采用盆栽,在每kg土壤施N和K2O各为0.033g基础上,设P1、P2、P3、P44个P处理(即每kg土壤分别施P2O50、0.033、0.067、0.100g)。采用紫外分光光度法测定不同大豆品种籽粒总异黄酮的含量。结果表明:同一品种P2处理大豆总异黄酮含量显著高于P1、P3、P4处理,‘黑农37’、‘黑农44’、‘黑农48’3个品种大豆P2处理大豆总异黄酮含量分别比对照组增加3.7%、4.3%、3.8%;不同品种同一处理都是‘黑农44’总异黄酮含量最高;在12个处理组合中‘黑农44’P2处理总异黄酮含量最高,3个大豆品种异黄酮含量在品种间和施磷处理间差异显著。施磷对3个大豆品种异黄酮含量有影响,适宜的施磷量有利于提高大豆籽粒异黄酮的含量。In this study,the effect of phosphorus on the content of kernal isoflavone in different soybean varieties was analyzed.The best phosphorus levels of different genotype soybean varieties were explored,in order to increase the content of kernal isoflavone and improve their qualities.Three soybean varieties（＇ Heinong-48 ＇,high-protein cultivar;＇ Heinong-37 ＇,middle cultivar;and ＇ Heinong-44 ＇,high-oil cultivar） were investigated.The soybean seeds were planted in pots and fertilized with 0.033 g of nitrogen（N） and potassium oxide（K 2 O） per kg soil.Four levels of phosphorus（P） treatment were designed,which were P 1,P 2,P 3 and P 4（i.e.,0,0.033,0.067,and 0.100 g of phosphorus pentoxide（P 2 O 5） per kilogram of soil）.The content of total kernal isoflavone in different soybean varieties was determined by ultraviolet spectrophotometry.The results showed that the content of total isoflavone under the P 2 treatment was significantly higher than that of P 1,P 3 and P 4 treatment in the same variety of soybean.The content of total isoflavone under the P 2 treatment in the three soybean varieties（＇ Heinong-37 ＇ ;＇ Heinong-44 ＇ ;＇ Heinong-48 ＇） compared to the control group was increased by 3.7%,4.3%,3.8%,respectively.It showed that the content of total isoflavone in ＇ Heinong-44 ＇ was the highest under the same treatment in different soybean varieties.In the 12 treatment combinations,the content of total isoflavone in ＇ Heinong-44 ＇ was the highest under the P 2 treatment.Significant differences were observed in the content of isoflavone between the varieties and levels of P treatments.These findings suggest that phosphorus affects the total content of isoflavone in the three soybean varieties,and appropriate amounts of P fertilization might contribute to improving the content of kernal isoflavone.

关 键 词：大豆 磷素水平 异黄酮 

分 类 号：S181[农业科学—农业基础科学]