卵巢癌病人腹水细胞的培养及其分化研究  

Research on the Culture and Differentiation of Ovarian Cancer Ascites Cells

作　　者：路巧然[1] 吴欣欣[1] 贾晓渊[1] 陈磊[1] 陈侃[1] 

机构地区：[1]浙江理工大学生命科学学院新元医学与生物技术研究所,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2011年第5期809-813,共5页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家自然科学基金项目(30801379);浙江省教育厅科研项目(20060593);浙江省重中之重学科开放基金项目(SWYX0812);浙江理工大学科研启动基金项目(0916819-Y)

摘　　要：肿瘤干细胞是指能分化出不同表型、自我更新和不断分化的肿瘤细胞群,具有与干细胞相似的特征。文章通过流式细胞术分析从人卵巢癌腹水分离所得的细胞表面蛋白的分化特征,发现这些腹水细胞具有部分干细胞表面标志(CD44+,CD90+,β2M+)蛋白,但却不表达CD133和CD34,且体外培养2周后,腹水细胞表面标志发生了变化,这些结果为卵巢癌干细胞研究提出了新的启示。Cancer stem cells（CSC）are cells that can differentiate the different phenotype,self-renewal and continued differentiation,also have similar characteristics with the stem cells.In this paper,by flow cytometry the authors analyze the differentiation characteristics of cell surface proteins that isolate from human ovarian cancer ascites,to find that these ascites cells have some stem cell surface marker（cluster of differentiation（CD） CD44＋,CD90＋）proteins,but don＇t have CD133 and CD34 proteins.After two weeks culture in vitro,there has some change in the markers of the ascites cells,these results raise new revelation for the research of ovarian cancer stem cell.

关 键 词：肿瘤干细胞 卵巢癌病人腹水细胞 表面标志 

分 类 号：R737.31[医药卫生—肿瘤]